WUSCHEL-RELATED HOMEOBOX 13 suppresses de novo shoot regeneration via cell fate control of pluripotent callus
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- Nao Ogura
- Division of Biological Sciences, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5, Takayama-cho, Ikoma, Nara 630-0192, Japan.
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- Yohei Sasagawa
- Department of Functional Genome Informatics, Division of Medical Genomics, Medical Research Institute, Tokyo Medical and Dental University, Bunkyo, Tokyo, Japan.
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- Tasuku Ito
- Department of Biology, Faculty of Science, Niigata University, Niigata, Niigata 950-2181, Japan.
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- Toshiaki Tameshige
- Division of Biological Sciences, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5, Takayama-cho, Ikoma, Nara 630-0192, Japan.
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- Satomi Kawai
- Department of Biology, Faculty of Science, Niigata University, Niigata, Niigata 950-2181, Japan.
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- Masaki Sano
- Department of Biology, Faculty of Science, Niigata University, Niigata, Niigata 950-2181, Japan.
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- Yuki Doll
- Division of Biological Sciences, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5, Takayama-cho, Ikoma, Nara 630-0192, Japan.
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- Akira Iwase
- RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.
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- Ayako Kawamura
- RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.
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- Takamasa Suzuki
- Department of Biological Chemistry, College of Biosciences and Biotechnology, Chubu University, Kasugai, Aichi 487-8501, Japan.
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- Itoshi Nikaido
- Department of Functional Genome Informatics, Division of Medical Genomics, Medical Research Institute, Tokyo Medical and Dental University, Bunkyo, Tokyo, Japan.
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- Keiko Sugimoto
- RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.
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- Momoko Ikeuchi
- Division of Biological Sciences, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5, Takayama-cho, Ikoma, Nara 630-0192, Japan.
Description
<jats:p> Plants can regenerate their bodies via de novo establishment of shoot apical meristems (SAMs) from pluripotent callus. Only a small fraction of callus cells is eventually specified into SAMs but the molecular mechanisms underlying fate specification remain obscure. The expression of WUSCHEL (WUS) is an early hallmark of SAM fate acquisition. Here, we show that a WUS paralog, WUSCHEL-RELATED HOMEOBOX 13 (WOX13), negatively regulates SAM formation from callus in <jats:italic>Arabidopsis thaliana</jats:italic> . WOX13 promotes non-meristematic cell fate via transcriptional repression of <jats:italic>WUS</jats:italic> and other SAM regulators and activation of cell wall modifiers. Our Quartz-Seq2–based single cell transcriptome revealed that WOX13 plays key roles in determining cellular identity of callus cell population. We propose that reciprocal inhibition between WUS and WOX13 mediates critical cell fate determination in pluripotent cell population, which has a major impact on regeneration efficiency. </jats:p>
Journal
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- Science Advances
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Science Advances 9 (27), 2023-07-07
American Association for the Advancement of Science (AAAS)
