Proposal of recommended experimental protocols for <i>in vitro</i> and <i>in vivo</i> evaluation methods of boron agents for neutron capture therapy

  • Yoshihide Hattori
    Research Center for BNCT, Osaka Metropolitan University , 1-1 Gakuen-cho, Nakaku, Sakai 599-8531 , Japan
  • Tooru Andoh
    Laboratory of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Kobe Gakuin University , Kobe 650-8586 , Japan
  • Shinji Kawabata
    Department of Neurosurgery, Osaka Medical and Pharmaceutical University , 2-7 Daigaku-machi, Takatsuki-shi, Osaka 569-8686 , Japan
  • Naonori Hu
    Kansai BNCT Medical Center, Osaka Medical and Pharmaceutical University , 2-7 Daigaku-machi, Takatsuki-shi, Osaka 569-8686 , Japan
  • Hiroyuki Michiue
    Neutron Therapy Research Center, Okayama University , 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558 , Japan
  • Hiroyuki Nakamura
    Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology , 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8503 , Japan
  • Takahiro Nomoto
    Department of Life Sciences , Graduate School of Arts and Sciences, , 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902 , Japan
  • Minoru Suzuki
    Institute for Integrated Radiation and Nuclear Science, Kyoto University , 2, Asashiro-Nishi, Kumatori-cho, Sennan-gun 590-0494 Japan
  • Takushi Takata
    Institute for Integrated Radiation and Nuclear Science, Kyoto University , 2, Asashiro-Nishi, Kumatori-cho, Sennan-gun 590-0494 Japan
  • Hiroki Tanaka
    Institute for Integrated Radiation and Nuclear Science, Kyoto University , 2, Asashiro-Nishi, Kumatori-cho, Sennan-gun 590-0494 Japan
  • Tsubasa Watanabe
    Institute for Integrated Radiation and Nuclear Science, Kyoto University , 2, Asashiro-Nishi, Kumatori-cho, Sennan-gun 590-0494 Japan
  • Koji Ono
    Kansai BNCT Medical Center, Osaka Medical and Pharmaceutical University , 2-7 Daigaku-machi, Takatsuki-shi, Osaka 569-8686 , Japan

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<jats:title>Abstract</jats:title> <jats:p>Recently, boron neutron capture therapy (BNCT) has been attracting attention as a minimally invasive cancer treatment. In 2020, the accelerator-based BNCT with L-BPA (Borofalan) as its D-sorbitol complex (Steboronine®) for head and neck cancers was approved by Pharmaceutical and Medical Devices Agency for the first time in the world. As accelerator-based neutron generation techniques are being developed in various countries, the development of novel tumor-selective boron agents is becoming increasingly important and desired. The Japanese Society of Neutron Capture Therapy believes it is necessary to propose standard evaluation protocols at each stage in the development of boron agents for BNCT. This review summarizes recommended experimental protocols for in vitro and in vivo evaluation methods of boron agents for BNCT based on our experience with L-BPA approval.</jats:p>

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