Protocol: an improved method for inducing sporophyte generation in the model moss Physcomitrium patens under nitrogen starvation
説明
<jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p>Land plants exhibit a haplodiplontic life cycle, whereby multicellular bodies develop in both the haploid and diploid generations. The early-diverging land plants, known as bryophytes, have a haploid-dominant life cycle, in which a short-lived multicellular body in the diploid generation, known as the sporophyte, develops on the maternal haploid gametophyte tissues. The moss <jats:italic>Physcomitrium</jats:italic> (<jats:italic>Physcomitrella</jats:italic>) <jats:italic>patens</jats:italic> has become one of the most powerful model systems in evolutionary plant developmental studies. To induce diploid sporophytes of <jats:italic>P. paten</jats:italic>s, several protocols are implemented. One of the conventional approaches is to grow approximately one-month-old gametophores for another month on Jiffy-7 pellets made from the peat moss that is difficult to fully sterilize. A more efficient method to obtain all tissues throughout the life cycle should accelerate studies of <jats:italic>P. paten</jats:italic>s.</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>Here, we investigated the effect of nitrogen conditions on the growth and development of <jats:italic>P. patens</jats:italic>. We provide an improved protocol for the sporophyte induction of <jats:italic>P. patens</jats:italic> using a BCD-based solid culture medium without Jiffy-7 pellets, based on the finding that the formation of gametangia and subsequent sporophytes is promoted by nitrogen-free growth conditions. The protocol consists of two steps; first, culture the protonemata and gametophores on nitrogen-rich medium under continuous light at 25 °C, and then transfer the gametophores onto nitrogen-free medium under short-day and at 15 °C for sporophyte induction. The protocol enables to shorten the induction period and reduce the culture space.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusions</jats:title> <jats:p>Our more efficient and shortened protocol for inducing the formation of sporophytes will contribute to future studies into the fertilization or the diploid sporophyte generation of <jats:italic>P. patens</jats:italic>.</jats:p> </jats:sec>
収録刊行物
-
- Plant Methods
-
Plant Methods 19 (1), 2023-09-26
Springer Science and Business Media LLC
