Identification and analysis of sugar transporters capable of co‐transporting glucose and xylose simultaneously

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  • Nurzhan Kuanyshev
    DOE Center for Advanced Bioenergy and Bioproducts Innovation University of Illinois at Urbana‐Champaign Urbana Illinois USA
  • Anshu Deewan
    DOE Center for Advanced Bioenergy and Bioproducts Innovation University of Illinois at Urbana‐Champaign Urbana Illinois USA
  • Sujit Sadashiv Jagtap
    DOE Center for Advanced Bioenergy and Bioproducts Innovation University of Illinois at Urbana‐Champaign Urbana Illinois USA
  • Jingjing Liu
    DOE Center for Advanced Bioenergy and Bioproducts Innovation University of Illinois at Urbana‐Champaign Urbana Illinois USA
  • Balaji Selvam
    Department of Chemical and Biomolecular Engineering University of Illinois at Urbana‐Champaign Urbana Illinois USA
  • Li‐Qing Chen
    DOE Center for Advanced Bioenergy and Bioproducts Innovation University of Illinois at Urbana‐Champaign Urbana Illinois USA
  • Diwakar Shukla
    Department of Chemical and Biomolecular Engineering University of Illinois at Urbana‐Champaign Urbana Illinois USA
  • Christopher V. Rao
    DOE Center for Advanced Bioenergy and Bioproducts Innovation University of Illinois at Urbana‐Champaign Urbana Illinois USA
  • Yong‐Su Jin
    DOE Center for Advanced Bioenergy and Bioproducts Innovation University of Illinois at Urbana‐Champaign Urbana Illinois USA

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<jats:title>Abstract</jats:title><jats:sec><jats:label /><jats:p>Simultaneous co‐fermentation of glucose and xylose is a key desired trait of engineered <jats:italic>Saccharomyces cerevisiae</jats:italic> for efficient and rapid production of biofuels and chemicals. However, glucose strongly inhibits xylose transport by endogenous hexose transporters of <jats:italic>S. cerevisiae</jats:italic>. We identified structurally distant sugar transporters (<jats:italic>Lipomyces starkeyi</jats:italic> LST1_205437 and <jats:italic>Arabidopsis thaliana At</jats:italic>SWEET7) capable of co‐transporting glucose and xylose from previously unexplored oleaginous yeasts and plants. Kinetic analysis showed that LST1_205437 had lenient glucose inhibition on xylose transport and <jats:italic>At</jats:italic>SWEET7 transported glucose and xylose simultaneously with no inhibition. Modelling studies of LST1_205437 revealed that Ala335 residue at sugar binding site can accommodates both glucose and xylose. Docking studies with <jats:italic>At</jats:italic>SWEET7 revealed that Trp59, Trp183, Asn145, and Asn179 residues stabilized the interactions with sugars, allowing both xylose and glucose to be co‐transported. In addition, we altered sugar preference of LST1_205437 by single amino acid mutation at Asn365. Our findings provide a new mechanistic insight on glucose and xylose transport mechanism of sugar transporters and the identified sugar transporters can be employed to develop engineered yeast strains for producing cellulosic biofuels and chemicals.</jats:p></jats:sec>

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