Chitin perception in plasmodesmata characterizes submembrane immune-signaling specificity in plants
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- Cécilia Cheval
- Crop Genetics, John Innes Centre, NR4 7UH Norwich, United Kingdom;
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- Sebastian Samwald
- Crop Genetics, John Innes Centre, NR4 7UH Norwich, United Kingdom;
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- Matthew G. Johnston
- Crop Genetics, John Innes Centre, NR4 7UH Norwich, United Kingdom;
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- Jeroen de Keijzer
- Crop Genetics, John Innes Centre, NR4 7UH Norwich, United Kingdom;
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- Andrew Breakspear
- Crop Genetics, John Innes Centre, NR4 7UH Norwich, United Kingdom;
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- Xiaokun Liu
- Crop Genetics, John Innes Centre, NR4 7UH Norwich, United Kingdom;
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- Annalisa Bellandi
- Crop Genetics, John Innes Centre, NR4 7UH Norwich, United Kingdom;
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- Yasuhiro Kadota
- The Sainsbury Laboratory, University of East Anglia, NR4 7UH Norwich, United Kingdom;
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- Cyril Zipfel
- The Sainsbury Laboratory, University of East Anglia, NR4 7UH Norwich, United Kingdom;
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- Christine Faulkner
- Crop Genetics, John Innes Centre, NR4 7UH Norwich, United Kingdom;
Abstract
<jats:p>The plasma membrane (PM) is composed of heterogeneous subdomains, characterized by differences in protein and lipid composition. PM receptors can be dynamically sorted into membrane domains to underpin signaling in response to extracellular stimuli. In plants, the plasmodesmal PM is a discrete microdomain that hosts specific receptors and responses. We exploited the independence of this PM domain to investigate how membrane domains can independently integrate a signal that triggers responses across the cell. Focusing on chitin signaling, we found that responses in the plasmodesmal PM require the LysM receptor kinases LYK4 and LYK5 in addition to LYM2. Chitin induces dynamic changes in the localization, association, or mobility of these receptors, but only LYM2 and LYK4 are detected in the plasmodesmal PM. We further uncovered that chitin-induced production of reactive oxygen species and callose depends on specific signaling events that lead to plasmodesmata closure. Our results demonstrate that distinct membrane domains can integrate a common signal with specific machinery that initiates discrete signaling cascades to produce a localized response.</jats:p>
Journal
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 117 (17), 9621-9629, 2020-04-13
Proceedings of the National Academy of Sciences
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Details 詳細情報について
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- CRID
- 1361131418768457600
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- ISSN
- 10916490
- 00278424
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- Data Source
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- Crossref
- KAKEN