Cartilaginous tissue formation from bone marrow cells using rotating wall vessel (RWV) bioreactor
書誌事項
- 公開日
- 2006-09-19
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1002/bit.20892
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p>This is the first successful report of the rapid regeneration of three‐dimensional large and homogeneous cartilaginous tissue from rabbit bone marrow cells without a scaffold using a rotating wall vessel (RWV) bioreactor, which simulates a microgravity environment for cells. Bone marrow cells cultured for 3 weeks in DMEM were resuspended and cultured for 4 weeks in the chondrogenic medium within the vessel. Large cylindrical cartilaginous tissue with dimensions of (1.25 ± 0.06) × (0.60 ± 0.08) cm (height × diameter) formed. Their cartilage marker expression was confirmed by mRNA expressions of aggrecan, collagen type I and II, and glycosaminoglycan (GAG)/DNA ratio. Their cartilaginous properties were demonstrated by toluidine blue, safranin‐O staining, and polarization. © 2006 Wiley Periodicals, Inc.</jats:p>
収録刊行物
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- Biotechnology and Bioengineering
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Biotechnology and Bioengineering 95 (5), 1003-1008, 2006-09-19
Wiley
