Very Small Embryonic-Like Stem Cells from the Murine Bone Marrow Differentiate into Epithelial Cells of the Lung
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- Susannah H. Kassmer
- Department of Laboratory Medicine, and Yale Flow Cytometry Core Facility, Yale University School of Medicine, New Haven, Connecticut, USA
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- Huiyan Jin
- Department of Laboratory Medicine, and Yale Flow Cytometry Core Facility, Yale University School of Medicine, New Haven, Connecticut, USA
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- Ping-Xia Zhang
- Department of Laboratory Medicine, and Yale Flow Cytometry Core Facility, Yale University School of Medicine, New Haven, Connecticut, USA
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- Emanuela M. Bruscia
- Department of Pediatrics, and Yale Flow Cytometry Core Facility, Yale University School of Medicine, New Haven, Connecticut, USA
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- Kartoosh Heydari
- Department of Immunobiology, Yale Flow Cytometry Core Facility, Yale University School of Medicine, New Haven, Connecticut, USA
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- Joo-Hyeon Lee
- Stem Cell Program, Division of Hematology/Oncology, Boston Children's Hospital, Boston, Massachusetts, USA
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- Carla F. Kim
- Stem Cell Program, Division of Hematology/Oncology, Boston Children's Hospital, Boston, Massachusetts, USA
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- Diane S. Krause
- Department of Laboratory Medicine, and Yale Flow Cytometry Core Facility, Yale University School of Medicine, New Haven, Connecticut, USA
書誌事項
- 公開日
- 2013-12-01
- 権利情報
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- https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model
- DOI
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- 10.1002/stem.1413
- 公開者
- Oxford University Press (OUP)
この論文をさがす
説明
<jats:title>Abstract</jats:title> <jats:p>The view that adult stem cells are lineage restricted has been challenged by numerous reports of bone marrow (BM)-derived cells giving rise to epithelial cells. Previously, we demonstrated that nonhematopoietic BM cells are the primary source of BM-derived lung epithelial cells. Here, we tested the hypothesis that very small embryonic like cells (VSELs) are responsible for this engraftment. We directly compared the level of BM-derived epithelial cells after transplantation of VSELs, hematopoietic stem/progenitor cells, or other nonhematopoietic cells. VSELs clearly had the highest rate of forming epithelial cells in the lung. By transplanting VSELs from donor mice expressing H2B-GFP under a type 2 pneumocyte-specific promoter, we demonstrate that this engraftment occurs by differentiation and not fusion. This is the first report of VSELs differentiating into an endodermal lineage in vivo, thereby potentially crossing germ layer lineages. Our data suggest that Oct4+ VSELs in the adult BM exhibit broad differentiation potential. STEM Cells 2013;31:2759–2766</jats:p>
収録刊行物
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- Stem Cells
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Stem Cells 31 (12), 2759-2766, 2013-12-01
Oxford University Press (OUP)