Re-epithelialization and immune cell behaviour in an ex vivo human skin model

DOI PDF Web Site 被引用文献93件

書誌事項

公開日
2020-01-08
権利情報
  • https://creativecommons.org/licenses/by/4.0
  • https://creativecommons.org/licenses/by/4.0
DOI
  • 10.1038/s41598-019-56847-4
公開者
Springer Science and Business Media LLC

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<jats:title>Abstract</jats:title><jats:p>A large body of literature is available on wound healing in humans. Nonetheless, a standardized<jats:italic>ex vivo</jats:italic>wound model without disruption of the dermal compartment has not been put forward with compelling justification. Here, we present a novel wound model based on application of negative pressure and its effects for epidermal regeneration and immune cell behaviour. Importantly, the basement membrane remained intact after blister roof removal and keratinocytes were absent in the wounded area. Upon six days of culture, the wound was covered with one to three-cell thick K14<jats:sup>+</jats:sup>Ki67<jats:sup>+</jats:sup>keratinocyte layers, indicating that proliferation and migration were involved in wound closure. After eight to twelve days, a multi-layered epidermis was formed expressing epidermal differentiation markers (K10, filaggrin, DSG-1, CDSN). Investigations about immune cell-specific manners revealed more T cells in the blister roof epidermis compared to normal epidermis. We identified several cell populations in blister roof epidermis and suction blister fluid that are absent in normal epidermis which correlated with their decrease in the dermis, indicating a dermal efflux upon negative pressure. Together, our model recapitulates the main features of epithelial wound regeneration, and can be applied for testing wound healing therapies and investigating underlying mechanisms.</jats:p>

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  • Scientific Reports

    Scientific Reports 10 (1), 1-, 2020-01-08

    Springer Science and Business Media LLC

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