<i>In situ</i> analysis of repair processes for oxidative DNA damage in mammalian cells
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- Li Lan
- Department of Molecular Genetics, Institute of Development, Aging, and Cancer, Tohoku University, Seiryomachi 4-1, Sendai 980-8575, Japan; Research Laboratory for Molecular Genetics, Yamagata University, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan; Biochemistry Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo 104-0045, Japan; and Laboratory of Structural Biology, National Institute on Environmental Health Sciences, National Institutes of Health, Research Triangle...
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- Satoshi Nakajima
- Department of Molecular Genetics, Institute of Development, Aging, and Cancer, Tohoku University, Seiryomachi 4-1, Sendai 980-8575, Japan; Research Laboratory for Molecular Genetics, Yamagata University, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan; Biochemistry Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo 104-0045, Japan; and Laboratory of Structural Biology, National Institute on Environmental Health Sciences, National Institutes of Health, Research Triangle...
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- Yoshitsugu Oohata
- Department of Molecular Genetics, Institute of Development, Aging, and Cancer, Tohoku University, Seiryomachi 4-1, Sendai 980-8575, Japan; Research Laboratory for Molecular Genetics, Yamagata University, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan; Biochemistry Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo 104-0045, Japan; and Laboratory of Structural Biology, National Institute on Environmental Health Sciences, National Institutes of Health, Research Triangle...
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- Masashi Takao
- Department of Molecular Genetics, Institute of Development, Aging, and Cancer, Tohoku University, Seiryomachi 4-1, Sendai 980-8575, Japan; Research Laboratory for Molecular Genetics, Yamagata University, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan; Biochemistry Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo 104-0045, Japan; and Laboratory of Structural Biology, National Institute on Environmental Health Sciences, National Institutes of Health, Research Triangle...
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- Satoshi Okano
- Department of Molecular Genetics, Institute of Development, Aging, and Cancer, Tohoku University, Seiryomachi 4-1, Sendai 980-8575, Japan; Research Laboratory for Molecular Genetics, Yamagata University, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan; Biochemistry Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo 104-0045, Japan; and Laboratory of Structural Biology, National Institute on Environmental Health Sciences, National Institutes of Health, Research Triangle...
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- Mitsuko Masutani
- Department of Molecular Genetics, Institute of Development, Aging, and Cancer, Tohoku University, Seiryomachi 4-1, Sendai 980-8575, Japan; Research Laboratory for Molecular Genetics, Yamagata University, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan; Biochemistry Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo 104-0045, Japan; and Laboratory of Structural Biology, National Institute on Environmental Health Sciences, National Institutes of Health, Research Triangle...
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- Samuel H. Wilson
- Department of Molecular Genetics, Institute of Development, Aging, and Cancer, Tohoku University, Seiryomachi 4-1, Sendai 980-8575, Japan; Research Laboratory for Molecular Genetics, Yamagata University, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan; Biochemistry Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo 104-0045, Japan; and Laboratory of Structural Biology, National Institute on Environmental Health Sciences, National Institutes of Health, Research Triangle...
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- Akira Yasui
- Department of Molecular Genetics, Institute of Development, Aging, and Cancer, Tohoku University, Seiryomachi 4-1, Sendai 980-8575, Japan; Research Laboratory for Molecular Genetics, Yamagata University, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan; Biochemistry Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo 104-0045, Japan; and Laboratory of Structural Biology, National Institute on Environmental Health Sciences, National Institutes of Health, Research Triangle...
書誌事項
- 公開日
- 2004-09-13
- DOI
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- 10.1073/pnas.0406048101
- 公開者
- National Academy of Sciences
この論文をさがす
説明
<jats:p> Oxidative DNA damage causes blocks and errors in transcription and replication, leading to cell death and genomic instability. Although repair mechanisms of the damage have been extensively analyzed <jats:italic>in vitro</jats:italic> , the actual <jats:italic>in vivo</jats:italic> repair processes remain largely unknown. Here, by irradiation with an UVA laser through a microscope lens, we have conditionally produced single-strand breaks and oxidative base damage at restricted nuclear regions of mammalian cells. We showed, in real time after irradiation by using antibodies and GFP-tagged proteins, rapid and ordered DNA repair processes of oxidative DNA damage in human cells. Furthermore, we characterized repair pathways by using repair-defective mammalian cells and found that DNA polymerase β accumulated at single-strand breaks and oxidative base damage by means of its 31- and 8-kDa domains, respectively, and that XRCC1 is essential for both polymerase β-dependent and proliferating cell nuclear antigen-dependent repair pathways of single-strand breaks. Thus, the repair of oxidative DNA damage is based on temporal and functional interactions among various proteins operating at the site of DNA damage in living cells. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 101 (38), 13738-13743, 2004-09-13
National Academy of Sciences
