Revisiting human natural killer cell subset function revealed cytolytic CD56 <sup>dim</sup> CD16 <sup>+</sup> NK cells as rapid producers of abundant IFN-γ on activation
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- Andrea De Maria
- Infectious Diseases Unit, Istituto Nazionale per la Ricerca sul Cancro, 16132 Genoa, Italy;
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- Federica Bozzano
- Istituto G. Gaslini, 16132 Genoa, Italy; and
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- Claudia Cantoni
- Istituto G. Gaslini, 16132 Genoa, Italy; and
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- Lorenzo Moretta
- Istituto G. Gaslini, 16132 Genoa, Italy; and
Description
<jats:p> The two major functions of human natural killer (NK) cells are conventionally associated with distinct cell subsets. Thus, cytolytic activity is mostly confined to the CD56 <jats:sup>dim</jats:sup> CD16 <jats:sup>+</jats:sup> subset, whereas cytokine production is generally assigned to CD56 <jats:sup>bright</jats:sup> CD16 <jats:sup>+/−</jats:sup> cells. In this study, we reevaluated the functional capabilities of these NK subsets with regard to the production of IFN-γ at different time points after cell triggering via NKp46 and NKp30 activating receptors. Different from previous studies, cytokine production was also assessed at early intervals. We show that CD56 <jats:sup>dim</jats:sup> NK cells produce IFN-γ already at 2 to 4 h, whereas no cytokine production is detected beyond 16 h. In contrast, CD56 <jats:sup>bright</jats:sup> cells release IFN-γ only at late time intervals (>16 h after stimulation). The rapid IFN-γ production by CD56 <jats:sup>dim</jats:sup> NK cells is in line with the presence of IFN-γ mRNA in freshly isolated cells. Rapid IFN-γ production was also induced by combinations of IL-2, IL-12, and IL-15. Our data indicate that not only cytolytic activity but also early IFN-γ production is a functional property of CD56 <jats:sup>dim</jats:sup> NK cells. Thus, this subset can assure a rapid and comprehensive NK cell intervention during the early phases of innate responses. </jats:p>
Journal
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 108 (2), 728-732, 2010-12-27
Proceedings of the National Academy of Sciences
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Details 詳細情報について
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- CRID
- 1361137043738097152
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- ISSN
- 10916490
- 00278424
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- Data Source
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- Crossref