Alcohol dehydrogenases that catalyse methyl formate synthesis participate in formaldehyde detoxification in the methylotrophic yeast <i>Candida boidinii</i>

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公開日
2004-03
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1002/yea.1101
公開者
Wiley

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<jats:title>Abstract</jats:title><jats:p>Methyl formate synthesis during growth on methanol by methylotrophic yeasts has been considered to play a role in formaldehyde detoxification. An enzyme that catalyses methyl formate synthesis was purified from methylotrophic yeasts, and was suggested to belong to a family of alcohol dehydrogenases (ADHs). In this study we report the gene cloning and gene disruption analysis of three ADH‐encoding genes in the methylotrophic yeast <jats:italic>Candida boidinii</jats:italic> (<jats:italic>CbADH1, CbADH2</jats:italic> and <jats:italic>CbADH3</jats:italic>) in order to clarify the physiological role of methyl formate synthesis. From the primary structures of these three genes, CbAdh1 was shown to be cytosolic and CbAdh2 and CbAdh3 were mitochondrial enzymes. Gene products of <jats:italic>CbADH1, CbADH2</jats:italic> and <jats:italic>CbADH3</jats:italic> expressed in <jats:italic>Escherichia coli</jats:italic> showed both ADH‐ and methyl formate‐synthesizing activities. The results of gene‐disruption analyses suggested that methyl formate synthesis was mainly catalysed by a cytosolic ADH (CbAdh1), and this enzyme contributed to formaldehyde detoxification through glutathione‐independent formaldehyde oxidation during growth on methanol by methylotrophic yeasts. The GenBank/EMBL/DDBJ Accession Nos for <jats:italic>CbADH1, CbADH2</jats:italic> and <jats:italic>CbADH3</jats:italic> are AB125900, AB125901 and AB125902, respectively. Copyright © 2004 John Wiley & Sons, Ltd.</jats:p>

収録刊行物

  • Yeast

    Yeast 21 (4), 341-350, 2004-03

    Wiley

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