RNA-peptide fusions for the <i>in vitro</i> selection of peptides and proteins

DOI Web Site 被引用文献133件
  • Richard W. Roberts
    Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114
  • Jack W. Szostak
    Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114

書誌事項

公開日
1997-11-11
DOI
  • 10.1073/pnas.94.23.12297
公開者
Proceedings of the National Academy of Sciences

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説明

<jats:p> Covalent fusions between an mRNA and the peptide or protein that it encodes can be generated by <jats:italic>in vitro</jats:italic> translation of synthetic mRNAs that carry puromycin, a peptidyl acceptor antibiotic, at their 3′ end. The stable linkage between the informational (nucleic acid) and functional (peptide) domains of the resulting joint molecules allows a specific mRNA to be enriched from a complex mixture of mRNAs based on the properties of its encoded peptide. Fusions between a synthetic mRNA and its encoded <jats:italic>myc</jats:italic> epitope peptide have been enriched from a pool of random sequence mRNA-peptide fusions by immunoprecipitation. Covalent RNA-peptide fusions should provide an additional route to the <jats:italic>in vitro</jats:italic> selection and directed evolution of proteins. </jats:p>

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