Frequent occurrence of deletions and duplications during somatic hypermutation: Implications for oncogene translocations and heavy chain disease
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- Tina Goossens
- Institute for Genetics, University of Cologne, 50931 Cologne, Germany
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- Ulf Klein
- Institute for Genetics, University of Cologne, 50931 Cologne, Germany
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- Ralf Küppers
- Institute for Genetics, University of Cologne, 50931 Cologne, Germany
書誌事項
- 公開日
- 1998-03-03
- DOI
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- 10.1073/pnas.95.5.2463
- 公開者
- Proceedings of the National Academy of Sciences
この論文をさがす
説明
<jats:p>Human naive and germinal center (GC) B cells were sorted by flow cytometry and rearranged V<jats:sub>H</jats:sub>region genes were amplified and sequenced from single cells. Whereas no deletions or insertions were found in naive B cells, ≈4% of in-frame and >40% of out-of-frame rearrangements of GC B cells harbored deletions and/or insertions of variable length. The pattern of deletions/insertions and their restriction to mutated V genes strongly suggests that they result from somatic hypermutation. Deletions and insertions account for ≈6% of somatic mutations introduced into rearranged V<jats:sub>H</jats:sub>region genes of GC B cells. These deletions/insertions seem to be the main cause for the generation of heavy chain disease proteins. Furthermore, it appears that several types of oncogene translocations (like c-myc translocations in Burkitt’s lymphoma) occur as a byproduct of somatic hypermutation within the GC—and not during V(D)J recombination in the bone marrow as previously thought.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 95 (5), 2463-2468, 1998-03-03
Proceedings of the National Academy of Sciences