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- Ivan Rehor
- Institute of Organic Chemistry and Biochemistry AS CR v.v.i. Flemingovo nam. 2 Prague 6 166 10 Czech Republic
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- Jitka Slegerova
- Institute of Organic Chemistry and Biochemistry AS CR v.v.i. Flemingovo nam. 2 Prague 6 166 10 Czech Republic
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- Jan Kucka
- Institute of Macromolecular Chemistry AS CR v.v.i. Heyrovského nám. 2 Prague 6 162 06 Czech Republic
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- Vladimir Proks
- Institute of Macromolecular Chemistry AS CR v.v.i. Heyrovského nám. 2 Prague 6 162 06 Czech Republic
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- Vladimira Petrakova
- Faculty of Biomedical Engineering Czech Technical University in Prague, Sítná sq. 3105, 272 01 Kladno (Czech Republic) and Institute of Physics AS CR v.v.i Prague 8 Czech Republic
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- Marie‐Pierre Adam
- Laboratoire de Photonique Quantique et Moléculaire UMR 8537 CNRS and ENS Cachan F‐94235 Cachan (France), Laboratoire Aimé Cotton, CNRS, Université Paris Sud and ENS Cachan F‐91405 Orsay France
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- François Treussart
- Laboratoire de Photonique Quantique et Moléculaire UMR 8537 CNRS and ENS Cachan F‐94235 Cachan (France), Laboratoire Aimé Cotton, CNRS, Université Paris Sud and ENS Cachan F‐91405 Orsay France
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- Stuart Turner
- EMAT, University of Antwerp Groenenborgerlaan 171 B‐2020 Antwerp Belgium
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- Sara Bals
- EMAT, University of Antwerp Groenenborgerlaan 171 B‐2020 Antwerp Belgium
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- Pavel Sacha
- Institute of Organic Chemistry and Biochemistry AS CR v.v.i. Flemingovo nam. 2 Prague 6 166 10 Czech Republic
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- Miroslav Ledvina
- Institute of Organic Chemistry and Biochemistry AS CR v.v.i. Flemingovo nam. 2 Prague 6 166 10 Czech Republic
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- Amy M. Wen
- Department of Biomedical Engineering Case Western Reserve University, School of Medicine and Engineering 10990 Euclid Avenue Cleveland Ohio USA
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- Nicole F. Steinmetz
- Department of Biomedical Engineering Case Western Reserve University, School of Medicine and Engineering 10990 Euclid Avenue Cleveland Ohio USA
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- Petr Cigler
- Institute of Organic Chemistry and Biochemistry AS CR v.v.i. Flemingovo nam. 2 Prague 6 166 10 Czech Republic
書誌事項
- 公開日
- 2014-02-05
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1002/smll.201302336
- 公開者
- Wiley
この論文をさがす
説明
<jats:p>High pressure high temperature (HPHT) nanodiamonds (NDs) represent extremely promising materials for construction of fluorescent nanoprobes and nanosensors. However, some properties of bare NDs limit their direct use in these applications: they precipitate in biological solutions, only a limited set of bio‐orthogonal conjugation techniques is available and the accessible material is greatly polydisperse in shape. In this work, we encapsulate bright 30‐nm fluorescent nanodiamonds (FNDs) in 10–20‐nm thick translucent (i.e., not altering FND fluorescence) silica shells, yielding monodisperse near‐spherical particles of mean diameter 66 nm. High yield modification of the shells with PEG chains stabilizes the particles in ionic solutions, making them applicable in biological environments. We further modify the opposite ends of PEG chains with fluorescent dyes or vectoring peptide using click chemistry. High conversion of this bio‐orthogonal coupling yielded circa 2000 dye or peptide molecules on a single FND. We demonstrate the superior properties of these particles by in vitro interaction with human prostate cancer cells: while bare nanodiamonds strongly aggregate in the buffer and adsorb onto the cell membrane, the shell encapsulated NDs do not adsorb nonspecifically and they penetrate inside the cells.</jats:p>
収録刊行物
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- Small
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Small 10 (6), 1106-1115, 2014-02-05
Wiley