A Plant Peptide Encoded by <i>CLV3</i> Identified by in Situ MALDI-TOF MS Analysis

DOI Web Site 71 Citations
  • Tatsuhiko Kondo
    Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.
  • Shinichiro Sawa
    Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.
  • Atsuko Kinoshita
    Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.
  • Satoko Mizuno
    Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.
  • Tatsuo Kakimoto
    Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.
  • Hiroo Fukuda
    Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.
  • Youji Sakagami
    Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.

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Description

<jats:p> The <jats:italic>Arabidopsis CLAVATA3</jats:italic> ( <jats:italic>CLV3</jats:italic> ) gene encodes a stem cell–specific protein presumed to be a precursor of a secreted peptide hormone. Matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS) applied to in situ <jats:italic>Arabidopsis</jats:italic> tissues determined the structure of a modified 12–amino acid peptide (MCLV3), which was derived from a conserved motif in the CLV3 sequence. Synthetic MCLV3 induced shoot and root meristem consumption as cells differentiated into other organs, displaying the typical phenotype of transgenic plants overexpressing <jats:italic>CLV3</jats:italic> . These results suggest that the functional peptide of CLV3 is MCLV3. </jats:p>

Journal

  • Science

    Science 313 (5788), 845-848, 2006-08-11

    American Association for the Advancement of Science (AAAS)

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