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    • Characterization of broadly pleiotropic phenotypes caused by an <i>hfq</i> insertion mutation in <i>Escherichia coli</i> K‐12

    DOI Web Site Web Site 被引用文献10件

    書誌事項

    公開日
    1994-07
    権利情報
    • http://onlinelibrary.wiley.com/termsAndConditions#vor
    DOI
    • 10.1111/j.1365-2958.1994.tb00400.x
    公開者
    Wiley

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    説明

    <jats:title>Summary</jats:title><jats:p>The region immediately downstream from the <jats:italic>miaA</jats:italic> tRNA modification gene at 94.8 min contains the <jats:italic>hfq</jats:italic> gene and the <jats:italic>hflA</jats:italic> region, which are important in the bacteriophage Qβ and lambda life cycles. The roles of these genes in bacteria remain largely unknown. We report here the characterization of two chromosomal <jats:italic>hfq</jats:italic> insertion mutations. An omega (ω) cassette insertion near the end of <jats:italic>hfq</jats:italic> resulted in phenotypes only slightly different from the parent, although transcript mapping demonstrated that the insertion was completely polar on <jats:italic>hfq</jats:italic> expression. In contrast, an equally polar omega cassette insertion near the beginning of <jats:italic>hfq</jats:italic> caused pronounced pleiotropic phenotypes, including decreased growth rates and yields, decreased negative supercoiling of piasmids in stationary phase, increased cell size, osmosensitivity, increased oxidation of carbon sources, increased sensitivity to ultraviolet light, and suppression of <jats:italic>bgl</jats:italic> activation by <jats:italic>hns</jats:italic> mutations, <jats:italic>hfq</jats:italic>::ω mutant phenotypes were distinct from those caused by omega insertions early in the <jats:italic>miaA</jats:italic> tRNA modification gene. On the other hand, both <jats:italic>hfq</jats:italic> insertions interfered with lambda phage plaque formation, probably by means of polarity at the <jats:italic>hflA</jats:italic> region. Together, these results show that <jats:italic>hfq</jats:italic> function plays a fundamental role in <jats:italic>Escherichia coli</jats:italic> physiology and that <jats:italic>hfq</jats:italic> and the <jats:italic>hflA</jats:italic> region are in the <jats:italic>amiB‐mutL‐miaA‐hfq‐hflX</jats:italic> superoperon.</jats:p>

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