Kinematics of flagellar swimming in <i>Euglena gracilis</i> : Helical trajectories and flagellar shapes

  • Massimiliano Rossi
    MathLab, International School for Advanced Studies, 34136 Trieste, Italy;
  • Giancarlo Cicconofri
    MathLab, International School for Advanced Studies, 34136 Trieste, Italy;
  • Alfred Beran
    Sezione Oceanografia, Istituto Nazionale di Oceanografia e di Geofisica Sperimentale, 34151 Trieste, Italy
  • Giovanni Noselli
    MathLab, International School for Advanced Studies, 34136 Trieste, Italy;
  • Antonio DeSimone
    MathLab, International School for Advanced Studies, 34136 Trieste, Italy;

書誌事項

公開日
2017-11-27
権利情報
  • https://creativecommons.org/licenses/by-nc-nd/4.0/
DOI
  • 10.1073/pnas.1708064114
公開者
Proceedings of the National Academy of Sciences

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説明

<jats:title>Significance</jats:title> <jats:p> Active flagella provide the propulsion mechanism for a large variety of swimming eukaryotic microorganisms, from protists to sperm cells. Planar and helical beating patterns of these structures are recurrent and widely studied. The fast spinning motion of the locomotory flagellum of the alga <jats:italic>Euglena gracilis</jats:italic> constitutes a remarkable exception to these patterns. We report a quantitative description of the 3D flagellar beating in swimming <jats:italic>E. gracilis</jats:italic> . Given their complexity, these shapes cannot be directly imaged with current microscopy techniques. We show how to overcome these limitations by developing a method to reconstruct in full the 3D kinematics of the cell from conventional 2D microscopy images, based on the exact characterization of the helical motion of the cell body. </jats:p>

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