Neurogenetic dissection of the Drosophila lateral horn reveals major outputs, diverse behavioural functions, and interactions with the mushroom body
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- Michael-John Dolan
- Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
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- Shahar Frechter
- Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
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- Alexander Shakeel Bates
- Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
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- Chuntao Dan
- Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
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- Paavo Huoviala
- Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
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- Ruairí JV Roberts
- Department of Zoology, University of Cambridge, Cambridge, United Kingdom
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- Philipp Schlegel
- Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
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- Serene Dhawan
- Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
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- Remy Tabano
- Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
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- Heather Dionne
- Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
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- Christina Christoforou
- Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
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- Kari Close
- Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
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- Ben Sutcliffe
- Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
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- Bianca Giuliani
- Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
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- Feng Li
- Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
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- Marta Costa
- Department of Zoology, University of Cambridge, Cambridge, United Kingdom
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- Gudrun Ihrke
- Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
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- Geoffrey Wilson Meissner
- Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
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- Davi D Bock
- Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
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- Yoshinori Aso
- Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
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- Gerald M Rubin
- Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
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- Gregory SXE Jefferis
- Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
書誌事項
- 公開日
- 2019-05-21
- 権利情報
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- http://creativecommons.org/licenses/by/4.0/
- http://creativecommons.org/licenses/by/4.0/
- http://creativecommons.org/licenses/by/4.0/
- DOI
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- 10.7554/elife.43079
- 公開者
- eLife Sciences Publications, Ltd
説明
<jats:p>Animals exhibit innate behaviours to a variety of sensory stimuli including olfactory cues. In Drosophila, one higher olfactory centre, the lateral horn (LH), is implicated in innate behaviour. However, our structural and functional understanding of the LH is scant, in large part due to a lack of sparse neurogenetic tools for this region. We generate a collection of split-GAL4 driver lines providing genetic access to 82 LH cell types. We use these to create an anatomical and neurotransmitter map of the LH and link this to EM connectomics data. We find ~30% of LH projections converge with outputs from the mushroom body, site of olfactory learning and memory. Using optogenetic activation, we identify LH cell types that drive changes in valence behavior or specific locomotor programs. In summary, we have generated a resource for manipulating and mapping LH neurons, providing new insights into the circuit basis of innate and learned olfactory behavior.</jats:p>
収録刊行物
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- eLife
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eLife 8 2019-05-21
eLife Sciences Publications, Ltd
