Response of <i>E. faecalis</i> biofilms to different associations of auxiliary substances during root canal preparation: A confocal laser microscopy analysis

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  • Gabriela Luisa Hohscheidt
    Conservative Dentistry Department School of Dentistry, Federal University of Rio Grande do Sul (UFRGS) Av. Ramiro Barcelos, 2492, Porto Alegre/RS, Cep 90035‐003 Brazil
  • Daiana Elisabeth Böttcher
    Conservative Dentistry Department School of Dentistry, Federal University of Rio Grande do Sul (UFRGS) Av. Ramiro Barcelos, 2492, Porto Alegre/RS, Cep 90035‐003 Brazil
  • Clarissa Cavalcanti Fatturi Parolo
    Social and Preventive Dentistry Department School of Dentistry, Federal University of Rio Grande do Sul (UFRGS) Av. Ramiro Barcelos, 2492, Porto Alegre /RS, Cep 90035‐003 Brazil
  • Francisco Montagner
    Conservative Dentistry Department School of Dentistry, Federal University of Rio Grande do Sul (UFRGS) Av. Ramiro Barcelos, 2492, Porto Alegre/RS, Cep 90035‐003 Brazil
  • Fabiana Soares Grecca
    Conservative Dentistry Department School of Dentistry, Federal University of Rio Grande do Sul (UFRGS) Av. Ramiro Barcelos, 2492, Porto Alegre/RS, Cep 90035‐003 Brazil

説明

<jats:title>ABSTRACT</jats:title><jats:p><jats:italic>Objective</jats:italic>: This <jats:italic>in vitro</jats:italic> study evaluated the effect of different endodontic auxiliary chemical substances over <jats:italic>Enterococcus faecalis (Ef)</jats:italic> biofilm through confocal laser scanning microscopy (CLSM). <jats:italic>Methods</jats:italic>: Forty‐five bovine incisors were infected with <jats:italic>Ef</jats:italic> for 21 days. Teeth were divided into five groups: group 1: 2.5% NaOCl + EDTA, group 2: 2% CHX gel + EDTA, group 3: 2% CHX liquid + EDTA, group 4: 2.5% NaOCl + 2% CHX gel + EDTA, group 5: 2.5% NaOCl + 2% CHX liquid + EDTA and a negative and a positive control group (NCG; PCG). The samples were stained with SYTO9 and propidium iodide and analyzed by CLSM. Bacterial viability was quantitatively analyzed by the proportions of dead and live bacteria in the biofilm remnants. Scores were standardized according to the total bacterial load (TBL)—1: ≤25%, 2: >25 ≤50%, 3: >50 ≤75%, 4: >75% and debris—1: absence of debris; 2: presence of debris. Statistical analysis was carried out through the Kruskal–Wallis and the Fischer exact tests (<jats:italic>P</jats:italic> = 0.05). <jats:italic>Results</jats:italic>: No statistical differences were observed to CFU, debris and bacterial viability. <jats:italic>Conclusion</jats:italic>: None of the tested substances could completely eliminate <jats:italic>Ef</jats:italic> from the root canal space. <jats:italic>Microsc. Res. Tech. 76:658–662, 2013</jats:italic>. © 2013 Wiley Periodicals, Inc.</jats:p>

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