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- Kim D. Janda
- Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
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- Diane Schloeder
- Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
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- Stephen J. Benkovic
- Departnent of Chemistry, Pennsylvania State University, University Park, PA 16802.
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- Richard A. Lerner
- Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
書誌事項
- 公開日
- 1988-09-02
- DOI
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- 10.1126/science.3413482
- 公開者
- American Association for the Advancement of Science (AAAS)
この論文をさがす
説明
<jats:p>Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.</jats:p>
収録刊行物
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- Science
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Science 241 (4870), 1188-1191, 1988-09-02
American Association for the Advancement of Science (AAAS)
