Spore germination and the life cycle of <i>Frankia in vitro</i>

DOI Web Site 被引用文献2件

書誌事項

公開日
1989-08-01
権利情報
  • http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining
DOI
  • 10.1139/m89-133
公開者
Canadian Science Publishing

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説明

<jats:p> Bacterial spores of Frankia produced in defined culture media were collected by filtration after washing in amounts approximating 10<jats:sup>6</jats:sup> spores/mL. Frankia strains UFGCeI5 from Casuarina equisetifolia and UFGCgI1 from C. glauca showed spontaneous release of spores in culture; strains HFPCcI3 from C. cunninghamiana and HFPAllI1 from Allocasuarina lehmanniana showed low spore release in culture unless homogenized. Spore germination was tested on plates of agar nutrient media under different physical and chemical environments. Strain CeI5 showed about 15% germination within 2 days in a defined (BAP) medium with an optimum pH of 6.0–6.8 at 28–35 °C. Under these conditions, strain CcI3 germinated less than 0.5%. In a series of trials with increasingly complex media, strain CeI5 showed 75% spore germination in 3 days at 28 °C and pH 6.7 in the most complex medium tested. Additions of specific single organic compounds to BAP medium caused either strong inhibition or slight stimulation of spore germination. Frankia strains that showed spontaneous spore release germinated better than strains that did not release. Spore germination in Frankia strains is markedly influenced by their strain origin and by the physical and chemical environment in which they are placed.Key words: Casuarina, Frankia, life cycle, spore germination. </jats:p>

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