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- David S. Wilson
- Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114-2696;,
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- Jack W. Szostak
- Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114-2696;,
抄録
<jats:p>▪ Abstract In vitro selection allows rare functional RNA or DNA molecules to be isolated from pools of over 10<jats:sup>15</jats:sup>different sequences. This approach has been used to identify RNA and DNA ligands for numerous small molecules, and recent three-dimensional structure solutions have revealed the basis for ligand recognition in several cases. By selecting high-affinity and -specificity nucleic acid ligands for proteins, promising new therapeutic and diagnostic reagents have been identified. Selection experiments have also been carried out to identify ribozymes that catalyze a variety of chemical transformations, including RNA cleavage, ligation, and synthesis, as well as alkylation and acyl-transfer reactions and N-glycosidic and peptide bond formation. The existence of such RNA enzymes supports the notion that ribozymes could have directed a primitive metabolism before the evolution of protein synthesis. New in vitro protein selection techniques should allow for a direct comparison of the frequency of ligand binding and catalytic structures in pools of random sequence polynucleotides versus polypeptides.</jats:p>
収録刊行物
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- Annual Review of Biochemistry
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Annual Review of Biochemistry 68 (1), 611-647, 1999-06
Annual Reviews
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詳細情報 詳細情報について
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- CRID
- 1361137044687729152
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- NII論文ID
- 80011363801
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- ISSN
- 15454509
- 00664154
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- データソース種別
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