Two Bacterial Diterpene Synthases from <i>Allokutzneria albata</i> Produce Bonnadiene, Phomopsene, and Allokutznerene
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- Lukas Lauterbach
- Kekulé-Institut für Organische Chemie und Biochemie Rheinische Friedrich-Wilhelms-Universität Bonn Gerhard-Domagk-Straße 1 53121 Bonn Germany
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- Jan Rinkel
- Kekulé-Institut für Organische Chemie und Biochemie Rheinische Friedrich-Wilhelms-Universität Bonn Gerhard-Domagk-Straße 1 53121 Bonn Germany
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- Jeroen S. Dickschat
- Kekulé-Institut für Organische Chemie und Biochemie Rheinische Friedrich-Wilhelms-Universität Bonn Gerhard-Domagk-Straße 1 53121 Bonn Germany
説明
<jats:title>Abstract</jats:title><jats:p>Two diterpene synthases from <jats:italic>Allokutzneria albata</jats:italic> were studied for their products, resulting in the identification of the new compound bonnadiene from the first enzyme. Although phylogenetically unrelated to fungal phomopsene synthase, the second enzyme produced a mixture of phomopsene and a biosynthetically linked new compound, allokutznerene, as well as spiroviolene. Both enzymes were subjected to in‐depth mechanistic studies involving isotopic labelling experiments, metal‐cofactor variation, and site‐directed mutagenesis. Oxidation products of phomopsene and allokutznerene are also discussed.</jats:p>
収録刊行物
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- Angewandte Chemie International Edition
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Angewandte Chemie International Edition 57 (27), 8280-8283, 2018-06-06
Wiley
