Expansion microscopy of zebrafish for neuroscience and developmental biology studies
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- Limor Freifeld
- Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139;
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- Iris Odstrcil
- Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138;
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- Dominique Förster
- Department Genes–Circuits–Behavior, Max Planck Institute of Neurobiology, Martinsried 82152, Germany;
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- Alyson Ramirez
- Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138;
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- James A. Gagnon
- Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138;
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- Owen Randlett
- Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138;
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- Emma K. Costa
- Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139;
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- Shoh Asano
- Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139;
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- Orhan T. Celiker
- Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139;
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- Ruixuan Gao
- Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139;
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- Daniel A. Martin-Alarcon
- Department of Biological Engineering, MIT, Cambridge, MA 02139;
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- Paul Reginato
- Department of Biological Engineering, MIT, Cambridge, MA 02139;
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- Cortni Dick
- Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139;
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- Linlin Chen
- Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139;
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- David Schoppik
- Department of Otolaryngology, New York University School of Medicine, New York, NY 10016;
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- Florian Engert
- Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138;
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- Herwig Baier
- Department Genes–Circuits–Behavior, Max Planck Institute of Neurobiology, Martinsried 82152, Germany;
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- Edward S. Boyden
- Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139;
説明
<jats:title>Significance</jats:title> <jats:p>We explore the utility of expansion microscopy (ExM) in neuroscience and developmental biology using the zebrafish model. Regarding neuroscience studies, ExM enables the tracing of cellular processes in the zebrafish brain, as well as the imaging of synapses and their biomolecular content and organization. Regarding development, ExM enables the resolving of nuclear compartments, particularly nuclear invaginations and channels, and helps relate such cellular nanostructures to proteins of the cytoskeleton during embryogenesis.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 114 (50), E10799-, 2017-11-21
Proceedings of the National Academy of Sciences
