Differential contribution of EF‐hands to the Ca²⁺‐dependent activation in the plant two‐pore channel TPC1

<jats:title>Summary</jats:title><jats:p>Two‐pore channels (TPC) have been established as components of calcium signalling networks in plants and animals. In plants, TPC1 in the vacuolar membrane is gated open upon binding of calcium in a voltage‐dependent manner. Here, we analyzed the molecular mechanism of the Ca<jats:sup>2+</jats:sup>‐dependent activity of TPC1 from <jats:italic>Arabidopsis thaliana</jats:italic>, using site‐directed mutagenesis of its two canonical EF‐hands. Wild‐type TPC1 and TPC1‐D335A with a mutated first Ca<jats:sup>2+</jats:sup> ligand in EF‐hand 1 produced channels that retained their voltage‐ and Ca<jats:sup>2+</jats:sup>‐dependent gating characteristics, but were less sensitive at Ca<jats:sup>2+</jats:sup> concentrations <200 μ<jats:sc>m</jats:sc>. Additional mutation of the first Ca<jats:sup>2+</jats:sup> ligand in EF‐hand 2 resulted in silent TPC1‐D335A/D376A channels. Similarly, the single mutant TPC1‐D376A could not be activated up to 1 m<jats:sc>m</jats:sc> Ca<jats:sup>2+</jats:sup>, indicating that the second EF‐hand is essential for the Ca<jats:sup>2+</jats:sup>‐dependent channel gating. Molecular modeling suggests that EF‐hand 1 displays a low‐affinity Ca<jats:sup>2+</jats:sup>/Mg<jats:sup>2+</jats:sup>‐binding site, while EF‐hand 2 represents a high‐affinity Ca<jats:sup>2+</jats:sup>‐binding site. Together, our data prove that EF‐hand 2 is responsible for the Ca<jats:sup>2+</jats:sup>‐receptor characteristics of TPC1, while EF‐hand 1 is a structural site required to enable channel responses at physiological changes in Ca<jats:sup>2+</jats:sup> concentration.</jats:p>