Cloning and sequencing of a 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase gene involved in the degradation of gamma-hexachlorocyclohexane in Pseudomonas paucimobilis

DOI Web Site PubMed 被引用文献9件 オープンアクセス
  • Y Nagata
    Department of Agricultural Chemistry, University of Tokyo, Japan.
  • R Ohtomo
    Department of Agricultural Chemistry, University of Tokyo, Japan.
  • K Miyauchi
    Department of Agricultural Chemistry, University of Tokyo, Japan.
  • M Fukuda
    Department of Agricultural Chemistry, University of Tokyo, Japan.
  • K Yano
    Department of Agricultural Chemistry, University of Tokyo, Japan.
  • M Takagi
    Department of Agricultural Chemistry, University of Tokyo, Japan.

書誌事項

公開日
1994-06
権利情報
  • https://journals.asm.org/non-commercial-tdm-license
DOI
  • 10.1128/jb.176.11.3117-3125.1994
公開者
American Society for Microbiology

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説明

<jats:p>In Pseudomonas paucimobilis UT26, gamma-hexachlorocyclohexane (gamma-HCH) is converted to 2,5-dichloro-2,5-cyclohexadiene-1,4-diol (2,5-DDOL), which is then metabolized to 2,5-dichlorohydroquinone. Here, we isolated from the genomic library of UT26 two genes which expressed 2,5-DDOL dehydrogenase activity when they were transformed into P. putida and Escherichia coli. Both gene products had an apparent molecular size of 28 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The first gene, named linC, located separately from the two genes (linA and linB) which we had already cloned as genes involved in the gamma-HCH degradation. The other, named linX, located about 1 kb upstream of the linA gene encoding gamma-HCH dehydrochlorinase. A gamma-HCH degradation-negative mutant, named UT72, which lacked the whole linC gene but had the intact linX gene was isolated. The linC gene given in a plasmid could complement UT72. These results strongly suggest that the linC gene but not the linX gene is essential for the assimilation of gamma-HCH in UT26. Deduced amino acid sequences of LinC and LinX show homology to those of members of the short-chain alcohol dehydrogenase family.</jats:p>

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