ISG56/IFIT1 is primarily responsible for interferon-induced changes to patterns of parainfluenza virus type 5 transcription and protein synthesis
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- J. Andrejeva
- School of Biology, Centre for Biomolecular Sciences, BMS Building, North Haugh, University of St Andrews, St Andrews, Fife, KY16 9ST, UK
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- H. Norsted
- School of Biology, Centre for Biomolecular Sciences, BMS Building, North Haugh, University of St Andrews, St Andrews, Fife, KY16 9ST, UK
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- M. Habjan
- Kantonal Hospital St Gallen, Institute of Immunobiology, CH-9007 St Gallen, Switzerland
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- V. Thiel
- Kantonal Hospital St Gallen, Institute of Immunobiology, CH-9007 St Gallen, Switzerland
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- S. Goodbourn
- Division of Basic Medical Sciences, St George’s, University of London, London SW17 0RE, UK
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- R. E. Randall
- School of Biology, Centre for Biomolecular Sciences, BMS Building, North Haugh, University of St Andrews, St Andrews, Fife, KY16 9ST, UK
説明
<jats:p>Interferon (IFN) induces an antiviral state in cells that results in alterations of the patterns and levels of parainfluenza virus type 5 (PIV5) transcripts and proteins. This study reports that IFN-stimulated gene 56/IFN-induced protein with tetratricopeptide repeats 1 (ISG56/IFIT1) is primarily responsible for these effects of IFN. It was shown that treating cells with IFN after infection resulted in an increase in virus transcription but an overall decrease in virus protein synthesis. As there was no obvious decrease in the overall levels of cellular protein synthesis in infected cells treated with IFN, these results suggested that ISG56/IFIT1 selectively inhibits the translation of viral mRNAs. This conclusion was supported by <jats:italic>in vitro</jats:italic> translation studies. Previous work has shown that ISG56/IFIT1 can restrict the replication of viruses lacking a 2′-<jats:italic>O</jats:italic>-methyltransferase activity, an enzyme that methylates the 2′-hydroxyl group of ribose sugars in the 5′-cap structures of mRNA. However, the data in the current study strongly suggested that PIV5 mRNAs are methylated at the 2′-hydroxyl group and thus that ISG56/IFIT1 selectively inhibits the translation of PIV5 mRNA by some as yet unrecognized mechanism. It was also shown that ISG56/IFIT1 is primarily responsible for the IFN-induced inhibition of PIV5.</jats:p>
収録刊行物
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- Journal of General Virology
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Journal of General Virology 94 (1), 59-68, 2013-01-01
Microbiology Society
