The <i>Tribolium chitin synthase</i> genes <i>TcCHS1</i> and <i>TcCHS2</i> are specialized for synthesis of epidermal cuticle and midgut peritrophic matrix

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公開日
2005-09-12
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  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1111/j.1365-2583.2005.00576.x
公開者
Wiley

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<jats:title>Abstract</jats:title> <jats:p> Functional analysis of the two <jats:italic>chitin synthase</jats:italic> genes, <jats:italic>TcCHS1</jats:italic> and <jats:italic>TcCHS2</jats:italic> , in the red flour beetle, <jats:italic>Tribolium castaneum</jats:italic> , revealed unique and complementary roles for each gene. <jats:italic>TcCHS1‐</jats:italic> specific RNA interference (RNAi) disrupted all three types of moult (larval–larval, larval–pupal and pupal–adult) and greatly reduced whole‐body chitin content. Exon‐specific RNAi showed that splice variant 8a of <jats:italic>TcCHS1</jats:italic> was required for both the larval‐pupal and pupal‐adult moults, whereas splice variant 8b was required only for the latter. <jats:italic>TcCHS2</jats:italic> ‐specific RNAi had no effect on metamorphosis or on total body chitin content. However, RNAi‐mediated down‐regulation of <jats:italic>TcCHS2</jats:italic> , but not <jats:italic>TcCHS1</jats:italic> , led to cessation of feeding, a dramatic shrinkage in larval size and reduced chitin content in the midgut. </jats:p>

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