Inhibition of the <i>Aplysia</i> sensory neuron calcium current with dopamine and serotonin

DOI Web Site 1 Citations
  • Tyler W. Dunn
    Department Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada
  • Wayne S. Sossin
    Department Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada

Description

<jats:p> The inhibition of Aplysia pleural mechanosensory neuron synapses by dopamine and serotonin through activation of endogenous dopaminergic and expressed 5-HT<jats:sub>1Apl(a)/b</jats:sub> receptors, respectively, involves a reduction in action potential-associated calcium influx. We show that the inhibition of synaptic efficacy is downstream of the readily releasable pool, suggesting that inhibition is at the level of calcium secretion coupling, likely a result of the changes in the calcium current. Indeed, the inhibitory responses directly reduce a Ca<jats:sub>V</jats:sub>2-like calcium current in isolated sensory neurons. The inhibition of the calcium current is voltage independent as it is not affected by a strong depolarizing prepulse, consistent with other invertebrate Ca<jats:sub>V</jats:sub>2 calcium currents. Similar to voltage-independent inhibition of vertebrate nociceptors, inhibition was blocked with Src tyrosine kinase inhibitors. The data suggest a conserved mechanism by which G protein-coupled receptor activation can inhibit the Ca<jats:sub>V</jats:sub>2 calcium current in nociceptive neurons. </jats:p>

Journal

Citations (1)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top