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- Tsute Chen
- <!--label omitted: 1-->Department of Molecular Genetics, The Forsyth Institute, Boston, Massachusetts 02115,1 and
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- Koji Nakayama
- <!--label omitted: 2-->Department of Microbiology, Faculty of Dentistry, Nagasaki University, Nagasaki, Japan2
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- Lynn Belliveau
- <!--label omitted: 1-->Department of Molecular Genetics, The Forsyth Institute, Boston, Massachusetts 02115,1 and
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- Margaret J. Duncan
- <!--label omitted: 1-->Department of Molecular Genetics, The Forsyth Institute, Boston, Massachusetts 02115,1 and
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- J. D. Clements
- editor
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説明
<jats:title>ABSTRACT</jats:title> <jats:p> <jats:italic>Porphyromonas gingivalis</jats:italic> is one of the principal organisms associated with adult periodontitis. Bacterial surface proteins such as fimbriae and gingipain hemagglutinin domains have been implicated as adhesins that actuate colonization of epithelium lining the gingival sulcus. We investigated the genetics of <jats:italic>P. gingivalis</jats:italic> adhesion to monolayers of epithelial cells using wild-type and gingipain mutant strains. These experiments suggested that arginine-specific gingipain (Rgp) catalytic activity modulated adhesion. From the data obtained with <jats:italic>rgp</jats:italic> mutants, we constructed a working hypothesis predicting that attachment and detachment of <jats:italic>P. gingivalis</jats:italic> to epithelial cells were mediated by gingipain adhesin and Rgp catalytic domains, respectively. A membrane-based epithelial cell binding assay, used to locate adhesins in extracellular fractions of wild-type and mutant strains, recognized gingipain peptides as adhesins rather than fimbriae. We developed a capture assay that demonstrated the binding of gingipain adhesin peptides to oral epithelial cells. The adherence of fimbrillin to epithelial cells was detected after heat denaturation of cell fractions. The prediction that Rgp catalytic activities mediated detachment was substantiated when the high level of attachment of an <jats:italic>rgp</jats:italic> mutant was reduced in the presence of wild-type cell fractions that contained gingipain catalytic activities. </jats:p>
収録刊行物
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- Infection and Immunity
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Infection and Immunity 69 (5), 3048-3056, 2001-05
American Society for Microbiology
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詳細情報 詳細情報について
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- CRID
- 1361137045304151424
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- NII論文ID
- 30020829391
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- NII書誌ID
- AA00673732
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- ISSN
- 10985522
- 00199567
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