High fat diet reduces the expression of miRNA‐29b in heart and increases susceptibility of myocardium to ischemia/reperfusion injury

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  • Elaine Castilho Guedes
    Department of Anatomy Laboratory of Cell Biology and Functional Anatomy, Institute of Biomedical Sciences, University of Sao Paulo Sao Paulo Brazil
  • Ivson Bezerra da Silva
    Department of Anatomy Laboratory of Cell Biology and Functional Anatomy, Institute of Biomedical Sciences, University of Sao Paulo Sao Paulo Brazil
  • Vanessa Morais Lima
    Department of Anatomy Laboratory of Cell Biology and Functional Anatomy, Institute of Biomedical Sciences, University of Sao Paulo Sao Paulo Brazil
  • Juliane B. Miranda
    Department of Anatomy Laboratory of Cell Biology and Functional Anatomy, Institute of Biomedical Sciences, University of Sao Paulo Sao Paulo Brazil
  • Rudá P. Albuquerque
    Department of Anatomy Laboratory of Integrative Systems Biology, Institute of Biomedical Sciences, University of Sao Paulo Sao Paulo Brazil
  • Julio C. B. Ferreira
    Department of Anatomy Laboratory of Integrative Systems Biology, Institute of Biomedical Sciences, University of Sao Paulo Sao Paulo Brazil
  • Maria Luiza M. Barreto‐Chaves
    Department of Anatomy Laboratory of Cell Biology and Functional Anatomy, Institute of Biomedical Sciences, University of Sao Paulo Sao Paulo Brazil
  • Gabriela Placoná Diniz
    Department of Anatomy Laboratory of Cell Biology and Functional Anatomy, Institute of Biomedical Sciences, University of Sao Paulo Sao Paulo Brazil

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<jats:title>Abstract</jats:title><jats:p>Several studies have shown the role of microRNAs (miRNAs) in myocardial dysfunction in response to ischemia/reperfusion (I/R). In this study, we investigated the impact of high fat (HF) diet in the myocardial susceptibility to I/R injury, as well as in the expression of miRNA‐29b. Isolated heart experiments using the ex vivo Langendorff perfusion model were used to induce cardiac I/R injury. HF diet‐induced cardiac hypertrophy and impaired cardiac functional recovery after I/R. miRNA‐29b, which targets Col1, was reduced in the heart of HF diet‐fed mice, whereas the cardiac expression of Col1 was increased. In addition, hypoxia–reoxygenation (H/R) reduced the expression of miRNA‐29b in cardiomyoblasts cultures. However, the overexpression of miRNA‐29b in cardiomyoblasts reduced p53 mRNA levels and H/R injury, suggesting that downregulation of miRNA‐29b may be involved in I/R injury. Together, our findings suggest that the reduced expression of miRNA‐29b may be involved in the deteriorated cardiac functional recovery following I/R in obese mice.</jats:p>

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