Determination of Histamine in Tomatoes by Liquid Chromatography/Mass Spectrometry

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  • Elek Bolygo
    Zeneca Agrochemicals, Jealotts Hill Research Station, Bracknell, Berkshire RG42 6ET, UK
  • Paul A Cooper
    Zeneca Agrochemicals, Jealotts Hill Research Station, Bracknell, Berkshire RG42 6ET, UK
  • K Michael Jessop
    Zeneca Agrochemicals, Jealotts Hill Research Station, Bracknell, Berkshire RG42 6ET, UK
  • Frank Moffatt
    Zeneca Agrochemicals, Jealotts Hill Research Station, Bracknell, Berkshire RG42 6ET, UK

Abstract

<jats:title>Abstract</jats:title> <jats:p>The histamine level in tomato fruits and pastes was determined by 2 orthogonal techniques as a means of comparing accuracy. Close statistical agreement was found between assays for free histamine by capillary electrophoresis (with UV absorbance detection), and for the dansyl derivative by reversed-phase liquid chromatography (LC). Both techniques have adequate sensitivity for the analysis of endogenous histamine in tomatoes, but LC/electrospray tandem mass spectrometry was more sensitive by at least an order of magnitude, down to a level of 0.05 mg/kg.</jats:p>

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