High thermodynamic stability of parametrically designed helical bundles

  • Po-Ssu Huang
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
  • Gustav Oberdorfer
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
  • Chunfu Xu
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
  • Xue Y. Pei
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK.
  • Brent L. Nannenga
    Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
  • Joseph M. Rogers
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
  • Frank DiMaio
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
  • Tamir Gonen
    Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
  • Ben Luisi
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK.
  • David Baker
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.

書誌事項

公開日
2014-10-24
DOI
  • 10.1126/science.1257481
公開者
American Association for the Advancement of Science (AAAS)

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説明

<jats:title>Building with alphahelical coiled coils</jats:title> <jats:p> Understanding how proteins fold into well-defined three-dimensional structures has been a longstanding challenge. Increased understanding has led to increased success at designing proteins that mimic existing protein folds. This raises the possibility of custom design of proteins with structures not seen in nature. Thomson <jats:italic>et al.</jats:italic> describe the design of channelcontaining α-helical barrels, and Huang <jats:italic>et al.</jats:italic> designed hyperstable helical bundles. Both groups used rational and computational design to make new protein structures based on α-helical coiled coils but took different routes to reach different target structures. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6208" page="485" related-article-type="in-this-issue" vol="346" xlink:href="10.1126/science.1257452">485</jats:related-article> , p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6208" page="481" related-article-type="in-this-issue" vol="346" xlink:href="10.1126/science.1257481">481</jats:related-article> </jats:p>

収録刊行物

  • Science

    Science 346 (6208), 481-485, 2014-10-24

    American Association for the Advancement of Science (AAAS)

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