THE ROLE OF CYTOKININS IN THE “FASCIATION” DISEASE CAUSED BY CORYNEBACTERIUM FASCIANS
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- Kenneth V. Thimann
- Biological Laboratories Harvard University Cambridge Massachusetts
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- Tsvi Sachs
- Biological Laboratories Harvard University Cambridge Massachusetts
説明
<jats:p>The disease caused by <jats:italic>Corynebacterium fascians</jats:italic> can be imitated by treatment with kinetin. Cultures of the bacteria were grown on a purine‐free medium of known composition. They require thiamine. Two assays for cytokinin were developed; one depends on the release of lateral pea buds from apical dominance and is highly specific but of only moderate sensitivity. The other depends on the retention of chlorophyll in senescing oat leaves and is very sensitive (detecting 2 × 10<jats:sup>–4</jats:sup> <jats:italic>μ</jats:italic>g of kinetin equivalents), though it is somewhat less specific and, therefore, requires care in usage. Both give results in 3–4 days and are thus far more rapid than tissue culture methods. With these assays <jats:italic>C. fascians</jats:italic> has been shown to produce a chloroform‐soluble cytokinin active in both tests. The substance is stable to heat in acid or basic media, is soluble in non‐polar solvents, and behaves as a base. It is precipitated from water solution by Ag ions and may, therefore, be a purine derivative. Pea tissue infected with <jats:italic>C. fascians</jats:italic>, but not uninfected tissue, yields a compound of similar solubilities and biological activity. Reasons are given for believing that the synthesis of cytokinins may be important, not only for <jats:italic>C. fascians</jats:italic>, but also for many other plant parasites.</jats:p>
収録刊行物
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- American Journal of Botany
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American Journal of Botany 53 (7), 731-739, 1966-08
Wiley
