Sensitivity of Polymerase Chain Reaction Assay for <i>Rickettsia tsutsugamushi</i> in Patients' Blood Samples

DOI Web Site Web Site 被引用文献1件
  • Koichi Murai
    Second Department of Medicine Miyazaki Medical School Miyazaki 889‐16 Japan
  • Nobuyoshi Tachibana
    Second Department of Medicine Miyazaki Medical School Miyazaki 889‐16 Japan
  • Akihiko Okayama
    Second Department of Medicine Miyazaki Medical School Miyazaki 889‐16 Japan
  • Eiichi Shishime
    Second Department of Medicine Miyazaki Medical School Miyazaki 889‐16 Japan
  • Kazunori Tsuda
    Second Department of Medicine Miyazaki Medical School Miyazaki 889‐16 Japan
  • Tatsumi Oshikawa
    Oshikawa Hospital Miyazaki 886‐02 Japan

書誌事項

公開日
1992-11
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1111/j.1348-0421.1992.tb02118.x
公開者
Wiley

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<jats:title>Abstract</jats:title><jats:p>We developed a nested polymerase chain reaction (PCR) method to detect <jats:italic>Rickettsia tsutsugamushi</jats:italic> (<jats:italic>R. tsutsugamushi</jats:italic>) DNA and determined its sensitivity. Primers were selected from the DNA sequence of the 58‐kDa group‐specific antigen gene of the Karp strain. The target sequence of rickettsial DNA was detectable as the band corresponding to 88 bp in 1.0 <jats:italic>μg</jats:italic> of the DNA extracted from BS‐C‐1 cells infected with <jats:italic>R. tsutsugamushi</jats:italic>. Rickettsia‐specific bands were observed not only for the homologous Karp strain, but also for four heterologous strains: two other reference strains (Gilliam and Kato) and two prototype strains prevalent in Miyazaki district (Irie and Hirano). The minimum copy number detectable by this method was estimated to be five rickettsiae. All of nine peripheral blood mononuclear cell samples from patients with tsutsugamushi disease who were seen 2‐11 days after disease onset tested positive for rickettsial DNA. The PCR assay method presented here could be a specific diagnostic tool for tsutsugamushi disease, especially in its early acute stage.</jats:p>

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