Tomato Root Penetration in Soil Requires a Coaction between Ethylene and Auxin Signaling

  • Parankusam Santisree
    School of Life Sciences, University of Hyderabad, Hyderabad 500046, India (P.S., S.N., H.V., Y.S., R.S.); Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331 (M.G.I.)
  • Sapana Nongmaithem
    School of Life Sciences, University of Hyderabad, Hyderabad 500046, India (P.S., S.N., H.V., Y.S., R.S.); Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331 (M.G.I.)
  • Himabindu Vasuki
    School of Life Sciences, University of Hyderabad, Hyderabad 500046, India (P.S., S.N., H.V., Y.S., R.S.); Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331 (M.G.I.)
  • Yellamaraju Sreelakshmi
    School of Life Sciences, University of Hyderabad, Hyderabad 500046, India (P.S., S.N., H.V., Y.S., R.S.); Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331 (M.G.I.)
  • Maria G. Ivanchenko
    School of Life Sciences, University of Hyderabad, Hyderabad 500046, India (P.S., S.N., H.V., Y.S., R.S.); Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331 (M.G.I.)
  • Rameshwar Sharma
    School of Life Sciences, University of Hyderabad, Hyderabad 500046, India (P.S., S.N., H.V., Y.S., R.S.); Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331 (M.G.I.)

抄録

<jats:title>Abstract</jats:title> <jats:p>During seed germination, emerging roots display positive gravitropism and penetrate into the soil for nutrition and anchorage. Tomato (Solanum lycopersicum) seeds germinated in the presence of 1-methylcyclopropene (1-MCP), an inhibitor of ethylene action, failed to insert roots into Soilrite and grew in the air, forming loops. Time-lapse video imaging showed that 1-MCP-grown root tips retained positive gravitropism and made contact with the surface of Soilrite but failed to penetrate into the Soilrite. Time-course studies revealed that the effect of 1-MCP was most prominent when seed imbibition and germination were carried out in the continual presence of 1-MCP. Conversely, 1-MCP was ineffective when applied postgermination after penetration of roots in the Soilrite. Furthermore, treatment with 1-MCP caused a reduction in DR5::β-glucuronidase auxin-reporter activity and modified the expression of SlIAA3 and SlIAA9 transcripts, indicating interference with auxin signaling. The reduced ethylene perception mutant, Never-ripe, displayed decreased ability for root penetration, and the enhanced polar auxin transport mutant, polycotyledon, showed a nearly normal root penetration in the presence of 1-MCP, which could be reversed by application of auxin transport inhibitors. Our results indicate that during tomato seed germination, a coaction between ethylene and auxin is required for root penetration into the soil.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 156 (3), 1424-1438, 2011-05-12

    Oxford University Press (OUP)

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