Prediction of function for the polyprenyl transferase subgroup in the isoprenoid synthase superfamily
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- Frank H. Wallrapp
- Department of Pharmaceutical Chemistry, School of Pharmacy and
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- Jian-Jung Pan
- Department of Chemistry, University of Utah, Salt Lake City, UT 84112;
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- Gurusankar Ramamoorthy
- Department of Chemistry, University of Utah, Salt Lake City, UT 84112;
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- Daniel E. Almonacid
- California Institute for Quantitative Biomedical Research, University of California, San Francisco, CA 94158;
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- Brandan S. Hillerich
- Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461
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- Ronald Seidel
- Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461
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- Yury Patskovsky
- Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461
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- Patricia C. Babbitt
- California Institute for Quantitative Biomedical Research, University of California, San Francisco, CA 94158;
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- Steven C. Almo
- Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461
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- Matthew P. Jacobson
- Department of Pharmaceutical Chemistry, School of Pharmacy and
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- C. Dale Poulter
- Department of Chemistry, University of Utah, Salt Lake City, UT 84112;
書誌事項
- 公開日
- 2013-03-14
- DOI
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- 10.1073/pnas.1300632110
- 公開者
- Proceedings of the National Academy of Sciences
この論文をさがす
説明
<jats:title>Significance</jats:title> <jats:p>This paper reports a large-scale collaborative study of an approach for predicting the function of chain elongation prenyltransferases from genetic data. A diverse set of genes for enzymes in the isoprenoid synthase superfamily was identified for cloning, expression, X-ray structural analysis, and prediction of function by docking to homology models. Blind predictions, later verified biochemically, were accurate to within one isoprene unit for all but a few of the 74 enzymes studied, an extraordinarily high level of prediction given that the enzymes often give products whose chain lengths vary by one isoprene unit.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 110 (13), E1196-, 2013-03-14
Proceedings of the National Academy of Sciences