GPR155 Serves as a Predictive Biomarker for Hematogenous Metastasis in Patients with Gastric Cancer
書誌事項
- 公開日
- 2017-02-06
- 権利情報
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- https://creativecommons.org/licenses/by/4.0
- https://creativecommons.org/licenses/by/4.0
- DOI
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- 10.1038/srep42089
- 公開者
- Springer Science and Business Media LLC
説明
<jats:title>Abstract</jats:title><jats:p>The prognosis of patients with gastric cancer (GC) with hematogenous metastasis is dismal. Identification of biomarkers specific for hematogenous metastasis is required to develop personalized treatments that improve patients’ outcomes. Global expression profiling of GC tissues with synchronous hepatic metastasis without metastasis to the peritoneal cavity or distant lymph nodes was conducted using next-generation sequencing and identified the G protein-coupled receptor 155 (<jats:italic>GPR155</jats:italic>) as a candidate biomarker. <jats:italic>GPR155</jats:italic> transcription was suppressed in GC cell lines compared with a nontumorigenic cell line. DNA methylation of the <jats:italic>GPR155</jats:italic> promoter region was not detected, albeit 20% of GC cell lines harbored copy number loss at GPR155 locus. The expression levels of <jats:italic>GPR155</jats:italic> mRNA correlated inversely with those of <jats:italic>TWIST1</jats:italic> and <jats:italic>WNT5B</jats:italic>. Inhibition of <jats:italic>GPR155</jats:italic> expression increased the levels of <jats:italic>p-ERK1/2</jats:italic> and <jats:italic>p-STAT1</jats:italic>, significantly increased cell proliferation, and increased the invasiveness of a GC cell lines. <jats:italic>GPR155</jats:italic> mRNA levels in GC clinical samples correlated with hematogenous metastasis and recurrence. Multivariate analysis revealed that reduced expression of <jats:italic>GPR155</jats:italic> mRNA was an independent predictive marker of hematogenous metastasis. <jats:italic>GPR155</jats:italic> may represent a biomarker for diagnosing and predicting hematogenous metastasis of GC.</jats:p>
収録刊行物
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- Scientific Reports
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Scientific Reports 7 (1), 42089-, 2017-02-06
Springer Science and Business Media LLC