Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets
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- Shengbin Rao
- Research and Development Center NH Foods Ltd. Tsukuba Japan
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- Tatsuya Fujimura
- Research and Development Center NH Foods Ltd. Tsukuba Japan
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- Hitomi Matsunari
- Department of Life Sciences Laboratory of Development Engineering School of Agriculture, Meiji University Kawasaki Japan
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- Tetsushi Sakuma
- Department of Mathematical and Life Sciences Graduate School of Science, Hiroshima University Higashi‐Hiroshima Japan
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- Kazuaki Nakano
- Department of Life Sciences Laboratory of Development Engineering School of Agriculture, Meiji University Kawasaki Japan
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- Masahito Watanabe
- Department of Life Sciences Laboratory of Development Engineering School of Agriculture, Meiji University Kawasaki Japan
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- Yoshinori Asano
- Department of Life Sciences Laboratory of Development Engineering School of Agriculture, Meiji University Kawasaki Japan
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- Eri Kitagawa
- Research and Development Center NH Foods Ltd. Tsukuba Japan
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- Takashi Yamamoto
- Department of Mathematical and Life Sciences Graduate School of Science, Hiroshima University Higashi‐Hiroshima Japan
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- Hiroshi Nagashima
- Department of Life Sciences Laboratory of Development Engineering School of Agriculture, Meiji University Kawasaki Japan
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<jats:title>SUMMARY</jats:title><jats:sec><jats:label /><jats:p>Myostatin (<jats:italic>MSTN</jats:italic>) is a negative regulator of myogenesis, and disruption of its function causes increased muscle mass in various species. Here, we report the generation of <jats:italic>MSTN</jats:italic>‐knockout (KO) pigs using genome editing technology combined with somatic‐cell nuclear transfer (SCNT). Transcription activator‐like effector nuclease (TALEN) with non‐repeat‐variable di‐residue variations, called Platinum TALEN, was highly efficient in modifying genes in porcine somatic cells, which were then used for SCNT to create <jats:italic>MSTN</jats:italic> KO piglets. These piglets exhibited a double‐muscled phenotype, possessing a higher body weight and longissimus muscle mass measuring 170% that of wild‐type piglets, with double the number of muscle fibers. These results demonstrate that loss of <jats:italic>MSTN</jats:italic> increases muscle mass in pigs, which may help increase pork production for consumption in the future. <jats:italic>Mol. Reprod. Dev. 83: 61–70, 2016. © 2015 Wiley Periodicals, Inc</jats:italic>.</jats:p></jats:sec>
収録刊行物
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- Molecular Reproduction and Development
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Molecular Reproduction and Development 83 (1), 61-70, 2015-11-09
Wiley
