Simplicispira limi sp. nov., isolated from activated sludge

<jats:p>A Gram-negative, motile, rod-shaped bacterium, designated strain EMB325<jats:sup>T</jats:sup>, was isolated from activated sludge that performed enhanced biological phosphorus removal in a sequencing batch reactor. The predominant fatty acids of strain EMB325<jats:sup>T</jats:sup> were summed feature 3 (C<jats:sub>16 : 1</jats:sub> <jats:italic>ω</jats:italic>7<jats:italic>c</jats:italic> and/or iso-C<jats:sub>15 : 0</jats:sub> 2-OH), C<jats:sub>16 : 0</jats:sub>, C<jats:sub>18 : 1</jats:sub> <jats:italic>ω</jats:italic>7<jats:italic>c</jats:italic> and C<jats:sub>18 : 1</jats:sub> <jats:italic>ω</jats:italic>7<jats:italic>c</jats:italic> 11-methyl. The strain contained phosphatidylethanolamine and diphosphatidylglycerol as polar lipids. The G+C content of the genomic DNA was 63.3 mol%. The major quinone was Q-8. Phylogenetic analysis of 16S rRNA gene sequences showed that strain EMB325<jats:sup>T</jats:sup> formed a phyletic lineage with members of the genus <jats:italic>Simplicispira</jats:italic> and was most closely related to <jats:italic>Simplicispira psychrophila</jats:italic> LMG 5408<jats:sup>T</jats:sup> and <jats:italic>Simplicispira metamorpha</jats:italic> DSM 1837<jats:sup>T</jats:sup> with similarities of 98.1 and 97.9 %, respectively. Levels of DNA–DNA relatedness between strain EMB325<jats:sup>T</jats:sup> and <jats:italic>S. psychrophila</jats:italic> LMG 5408<jats:sup>T</jats:sup> and <jats:italic>S. metamorpha</jats:italic> DSM 1837<jats:sup>T</jats:sup> were 28 and 23 %, respectively. On the basis of chemotaxonomic data and molecular characteristics, strain EMB325<jats:sup>T</jats:sup> is considered to represent a novel species within the genus <jats:italic>Simplicispira</jats:italic>, for which the name <jats:italic>Simplicispira limi</jats:italic> sp. nov. is proposed. The type strain is EMB325<jats:sup>T</jats:sup> (=KCTC 12608<jats:sup>T</jats:sup>=DSM 17964<jats:sup>T</jats:sup>).</jats:p>