Rapid Method for Determining Alkaline Phosphatase Activity in Serum with Thymolphthalein Monophosphate
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- A V Roy
- Pathology Department, South Side Hospital, S. 20th and Jane Sts., Pittsburgh, Pa. 15203.
書誌事項
- 公開日
- 1970-05-01
- 権利情報
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- https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model
- DOI
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- 10.1093/clinchem/16.5.431
- 公開者
- Oxford University Press (OUP)
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説明
<jats:title>Abstract</jats:title> <jats:p>The use of sodium thymolphthalein monophosphate in measuring alkaline phosphatase activity of serum is described. Thymolphthalein liberated in the reaction is conveniently measured by raising the pH of the medium, which produces a color and also stops the hydrolysis. The effects of pH, kind and molarity of buffer, temperature, and enzyme and magnesium con-centrations were studied. Results by this procedure correlate well with those obtained by a standard procedure in which liberated p-nitrophenol is de-termined. Advantages of the present method include excellent linearity, high precision, short incubation, and reagent stability.</jats:p>
収録刊行物
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- Clinical Chemistry
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Clinical Chemistry 16 (5), 431-436, 1970-05-01
Oxford University Press (OUP)