Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold
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- Michael G. Poirier
- Departments of Physics and Bioengineering, University of Illinois, Chicago, IL 60607-7059
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- John F. Marko
- Departments of Physics and Bioengineering, University of Illinois, Chicago, IL 60607-7059
書誌事項
- 公開日
- 2002-11-18
- DOI
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- 10.1073/pnas.232442599
- 公開者
- Proceedings of the National Academy of Sciences
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説明
<jats:p> Isolated newt ( <jats:italic>Notophthalmus viridescens</jats:italic> ) chromosomes were studied by using micromechanical force measurement during nuclease digestion. Micrococcal nuclease and short-recognition-sequence blunt-cutting restriction enzymes first remove the native elastic response of, and then to go on to completely disintegrate, single metaphase newt chromosomes. These experiments rule out the possibility that the mitotic chromosome is based on a mechanically contiguous internal non-DNA (e.g., protein) “scaffold”; instead, the mechanical integrity of the metaphase chromosome is due to chromatin itself. Blunt-cutting restriction enzymes with longer recognition sequences only partially disassemble mitotic chromosomes and indicate that chromatin in metaphase chromosomes is constrained by isolated chromatin-crosslinking elements spaced by ≈15 kb. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 99 (24), 15393-15397, 2002-11-18
Proceedings of the National Academy of Sciences
