A versatile Multisite Gateway‐compatible promoter and transgenic line collection for cell type‐specific functional genomics in Arabidopsis
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- Maria Mar Marquès‐Bueno
- Laboratoire de Reproduction et Développement des Plantes UMR 5667 CNRS/INRA/ENS‐Lyon/Université de Lyon 46 allée d'Italie 69364 Lyon Cedex 07 France
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- Ana K. Morao
- Ecole Normale Supérieure Institut de Biologie de l'Ecole Normale Supérieure Paris F‐75005 France
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- Anne Cayrel
- Institute for Integrative Biology of the Cell UMR 9198 CNRS/CEA/University Paris‐Sud Avenue de la Terrasse 91190 Gif‐sur‐Yvette France
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- Matthieu P. Platre
- Laboratoire de Reproduction et Développement des Plantes UMR 5667 CNRS/INRA/ENS‐Lyon/Université de Lyon 46 allée d'Italie 69364 Lyon Cedex 07 France
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- Marie Barberon
- Department of Plant Molecular Biology UNIL‐Sorge University of Lausanne 1015 Lausanne Switzerland
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- Erwann Caillieux
- Ecole Normale Supérieure Institut de Biologie de l'Ecole Normale Supérieure Paris F‐75005 France
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- Vincent Colot
- Ecole Normale Supérieure Institut de Biologie de l'Ecole Normale Supérieure Paris F‐75005 France
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- Yvon Jaillais
- Laboratoire de Reproduction et Développement des Plantes UMR 5667 CNRS/INRA/ENS‐Lyon/Université de Lyon 46 allée d'Italie 69364 Lyon Cedex 07 France
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- François Roudier
- Ecole Normale Supérieure Institut de Biologie de l'Ecole Normale Supérieure Paris F‐75005 France
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- Grégory Vert
- Institute for Integrative Biology of the Cell UMR 9198 CNRS/CEA/University Paris‐Sud Avenue de la Terrasse 91190 Gif‐sur‐Yvette France
Abstract
<jats:title>Summary</jats:title><jats:p>Multicellular organisms are composed of many cell types that acquire their specific fate through a precisely controlled pattern of gene expression in time and space dictated in part by cell type‐specific promoter activity. Understanding the contribution of highly specialized cell types in the development of a whole organism requires the ability to isolate or analyze different cell types separately. We have characterized and validated a large collection of root cell type‐specific promoters and have generated cell type‐specific marker lines. These benchmarked promoters can be readily used to evaluate cell type‐specific complementation of mutant phenotypes, or to knockdown gene expression using targeted expression of artificial mi<jats:styled-content style="fixed-case">RNA</jats:styled-content>. We also generated vectors and characterized transgenic lines for cell type‐specific induction of gene expression and cell type‐specific isolation of nuclei for <jats:styled-content style="fixed-case">RNA</jats:styled-content> and chromatin profiling. Vectors and seeds from transgenic Arabidopsis plants will be freely available, and will promote rapid progress in cell type‐specific functional genomics. We demonstrate the power of this promoter set for analysis of complex biological processes by investigating the contribution of root cell types in the <jats:styled-content style="fixed-case">IRT</jats:styled-content>1‐dependent root iron uptake. Our findings revealed the complex spatial expression pattern of <jats:italic><jats:styled-content style="fixed-case">IRT</jats:styled-content>1</jats:italic> in both root epidermis and phloem companion cells and the requirement for <jats:italic><jats:styled-content style="fixed-case">IRT</jats:styled-content>1</jats:italic> to be expressed in both cell types for proper iron homeostasis.</jats:p>
Journal
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- The Plant Journal
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The Plant Journal 85 (2), 320-333, 2016-01
Wiley
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Details 詳細情報について
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- CRID
- 1361137046371546112
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- ISSN
- 1365313X
- 09607412
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- Data Source
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- Crossref