Defined DNA/nanoparticle conjugates

  • Christopher J. Ackerson
    Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305
  • Michael T. Sykes
    Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305
  • Roger D. Kornberg
    Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305

説明

<jats:p>Glutathione monolayer-protected gold clusters were reacted by place exchange with 19- or 20-residue thiolated oligonucleotides. The resulting DNA/nanoparticle conjugates could be separated on the basis of the number of bound oligonucleotides by gel electrophoresis and assembled with one another by DNA-DNA hybridization. This approach overcomes previous limitations of DNA/nanoparticle synthesis and yields conjugates that are precisely defined with respect to both gold and nucleic acid content.</jats:p>

収録刊行物

被引用文献 (3)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ