Transcriptomic Analysis of Breast Cancer Stem Cells and Development of a pALDH1A1:mNeptune Reporter System for Live Tracking
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- Nadège Bidan
- University of Lille U908‐CPAC, Cell Plasticity and Cancer F‐59000 Lille France
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- Justine Bailleul‐Dubois
- University of Lille U908‐CPAC, Cell Plasticity and Cancer F‐59000 Lille France
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- Jérémy Duval
- Institut National de la Santé et de la recherche Médicale (INSERM) U908 F‐59000 Lille France
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- Marie Winter
- University of Lille U908‐CPAC, Cell Plasticity and Cancer F‐59000 Lille France
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- Marie Denoulet
- University of Lille U908‐CPAC, Cell Plasticity and Cancer F‐59000 Lille France
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- Karine Hannebicque
- Institut National de la Santé et de la recherche Médicale (INSERM) U908 F‐59000 Lille France
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- Ihsan Y. El‐Sayed
- University of Lille U908‐CPAC, Cell Plasticity and Cancer F‐59000 Lille France
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- Christophe Ginestier
- Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la recherche Médicale (INSERM) Paoli‐Calmettes Institute Centre de Recherche en Cancérologie de Marseille (CRCM), Epithelial Stem Cells and Cancer Team University of Aix‐Marseille 13009 Marseille France
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- Violaine Forissier
- Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la recherche Médicale (INSERM) Paoli‐Calmettes Institute Centre de Recherche en Cancérologie de Marseille (CRCM), Epithelial Stem Cells and Cancer Team University of Aix‐Marseille 13009 Marseille France
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- Emmanuelle Charafe‐Jauffret
- Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la recherche Médicale (INSERM) Paoli‐Calmettes Institute Centre de Recherche en Cancérologie de Marseille (CRCM), Epithelial Stem Cells and Cancer Team University of Aix‐Marseille 13009 Marseille France
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- Manon Macario
- Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la recherche Médicale (INSERM) Paoli‐Calmettes Institute Centre de Recherche en Cancérologie de Marseille (CRCM), Epithelial Stem Cells and Cancer Team University of Aix‐Marseille 13009 Marseille France
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- Yukiko T Matsunaga
- Center for International Research on Integrative Biomedical Systems (CIBiS) Institute of Industrial Science The University of Tokyo 4‐6‐1 Komaba Meguro‐ku Tokyo 153‐8505 Japan
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- Samuel Meignan
- Institut National de la Santé et de la recherche Médicale (INSERM) U908 F‐59000 Lille France
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- François Anquez
- Laboratory of Physics of Lasers, Atoms and Molecules, UMR CNRS 8523 University of Lille Villeneuve‐d'Ascq 59655 France
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- Sylvain Julien
- University of Lille U908‐CPAC, Cell Plasticity and Cancer F‐59000 Lille France
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- Amélie Bonnefond
- CNRS UMR 8199 European Genomic Institute for Diabetes (EGID) Institut Pasteur de Lille University of Lille 59000 Lille France
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- Mehdi Derhourhi
- CNRS UMR 8199 European Genomic Institute for Diabetes (EGID) Institut Pasteur de Lille University of Lille 59000 Lille France
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- Xuefen Le Bourhis
- University of Lille U908‐CPAC, Cell Plasticity and Cancer F‐59000 Lille France
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- Chann Lagadec
- University of Lille U908‐CPAC, Cell Plasticity and Cancer F‐59000 Lille France
Description
<jats:title>Abstract</jats:title><jats:p>Many solid cancers are hierarchically organized with a small number of cancer stem cells (CSCs) able to regrow a tumor, while their progeny lacks this feature. Breast CSC is known to contribute to therapy resistance. The study of those cells is usually based on their cell‐surface markers like CD44<jats:sup>high</jats:sup>/CD24<jats:sup>low/neg</jats:sup> or their aldehyde dehydrogenase (ALDH) activity. However, these markers cannot be used to track the dynamics of CSC. Here, a transcriptomic analysis is performed to identify segregating gene expression in CSCs and non‐CSCs, sorted by Aldefluor assay. It is observed that among ALDH‐associated genes, only ALDH1A1 isoform is increased in CSCs. A CSC reporter system is then developed by using a far red‐fluorescent protein (mNeptune) under the control of ALDH1A1 promoter. mNeptune‐positive cells exhibit higher sphere‐forming capacity, tumor formation, and increased resistance to anticancer therapies. These results indicate that the reporter identifies cells with stemness characteristics. Moreover, live tracking of cells in a microfluidic system reveals a higher extravasation potential of CSCs. Live tracking of non‐CSCs under irradiation treatment show, for the first time, live reprogramming of non‐CSCs into CSCs. Therefore, the reporter will allow for cell tracking to better understand the implication of CSCs in breast cancer development and recurrence.</jats:p>
Journal
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- PROTEOMICS
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PROTEOMICS 19 (21-22), 2019-09-08
Wiley
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Details 詳細情報について
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- CRID
- 1361412889718942592
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- ISSN
- 16159861
- 16159853
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- Data Source
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- Crossref
- KAKEN