<i>In vitro</i> characterization of the human segmentation clock
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説明
<jats:p>The vertebral column is characterized by the periodic arrangement of vertebrae along the anterior-posterior (AP) axis. This segmental or metameric organization is established early in embryogenesis when pairs of embryonic segments called somites are rhythmically produced by the presomitic mesoderm (PSM). The tempo of somite formation is controlled by a molecular oscillator known as the segmentation clock <jats:sup>1,2</jats:sup>. While this oscillator has been well characterized in model organisms <jats:sup>1,2</jats:sup>, whether a similar oscillator exists in humans remains unknown. We have previously shown that human embryonic stem (ES) cells or induced pluripotent stem (iPS) cells can differentiate <jats:italic>in vitro</jats:italic> into PSM upon activation of the Wnt signaling pathway combined with BMP inhibition<jats:sup>3</jats:sup>. Here, we show that these human PSM cells exhibit Notch and YAP-dependent oscillations<jats:sup>4</jats:sup> of the cyclic gene <jats:italic>HES7</jats:italic> with a 5-hour period. Single cell RNA-sequencing comparison of the differentiating iPS cells with mouse PSM reveals that human PSM cells follow a similar differentiation path and exhibit a remarkably coordinated differentiation sequence. We also demonstrate that FGF signaling controls the phase and period of the oscillator. This contrasts with classical segmentation models such as the “Clock and Wavefront” <jats:sup>1,2,5</jats:sup>, where FGF merely implements a signaling threshold specifying where oscillations stop. Overall, our work identifying the human segmentation clock represents an important breakthrough for human developmental biology.</jats:p>
収録刊行物
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- Nature
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Nature 580 (7801), 113-118, 2018-11-04
Cold Spring Harbor Laboratory
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キーワード
- Male
- Pluripotent Stem Cells
- 571
- General Science & Technology
- 1.1 Normal biological development and functioning
- Cells
- Embryonic Development
- In Vitro Techniques
- Article
- Mice
- Underpinning research
- Biological Clocks
- Genetics
- 2.1 Biological and endogenous factors
- Animals
- Humans
- RNA-Seq
- Aetiology
- Cells, Cultured
- Multidisciplinary
- Cultured
- Cell Differentiation
- Fibroblast Growth Factors
- Somites
- Female
- Single-Cell Analysis
- Signal Transduction
詳細情報 詳細情報について
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- CRID
- 1360022306715886720
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- ISSN
- 14764687
- 00280836
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- PubMed
- 32720482
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE