Abnormal development of zebrafish after knockout and knockdown of ribosomal protein L10a
書誌事項
- 公開日
- 2019-12-02
- 資源種別
- journal article
- 権利情報
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- https://creativecommons.org/licenses/by/4.0
- https://creativecommons.org/licenses/by/4.0
- DOI
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- 10.1038/s41598-019-54544-w
- 10.60692/7g36n-61q10
- 10.60692/nbr26-ha882
- 公開者
- Springer Science and Business Media LLC
説明
<jats:title>Abstract</jats:title><jats:p>In this study, to investigate the secondary function of Rpl10a in zebrafish development, morpholino antisense oligonucleotides (MOs) were used to knock down the zebrafish ribosomal protein L10a (<jats:italic>rpl10a</jats:italic>). At 25 hpf (hours post-fertilization), embryos injected with the <jats:italic>rpl10a</jats:italic> MO showed an abnormal morphology, including short bodies, curved tails, and small yolk sac extensions. We observed pigment reductions, edema, larger yolk sacs, smaller eyes and smaller yolk sac extensions at 50 hpf. In addition, reductions in the expression of primordial germ cell (PGC) marker genes (<jats:italic>nanos1</jats:italic> and <jats:italic>vasa</jats:italic>) were observed in <jats:italic>rpl10a</jats:italic> knockdown embryos. A rescue experiment using a <jats:italic>rpl10a</jats:italic> mRNA co-injection showed the recovery of the morphology and red blood cell production similar to wild-type. Moreover, the CRISPR-Cas9 system was used to edit the sequence of <jats:italic>rpl10a</jats:italic> exon 5, resulting in a homozygous 5-bp deletion in the zebrafish genome. The mutant embryos displayed a morphology similar to that of the knockdown animals. Furthermore, the loss of <jats:italic>rpl10a</jats:italic> function led to reduced expression of <jats:italic>gata1</jats:italic>, <jats:italic>hbae3</jats:italic>, and <jats:italic>hbbe1</jats:italic> (erythroid synthesis) and increased <jats:italic>tp53</jats:italic> expression. Overall, the results suggested that Rpl10a deficiency caused delays in embryonic development, as well as apoptosis and anemia, in zebrafish.</jats:p>
収録刊行物
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- Scientific Reports
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Scientific Reports 9 (1), 2019-12-02
Springer Science and Business Media LLC
- Tweet
キーワード
- Cell biology
- Embryo, Nonmammalian
- Molecular biology
- Exon
- Morpholino
- Gene
- Article
- DEAD-box RNA Helicases
- Gene Knockout Techniques
- Hemoglobins
- Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated proteins
- GATA1
- Biochemistry, Genetics and Molecular Biology
- Genetics
- Ribosomal protein
- Animals
- Erythropoiesis
- GATA1 Transcription Factor
- Sex Determination and Differentiation in Organisms
- Molecular Biology
- Biology
- Zebrafish
- mRNA modification
- Gene knockdown
- Gene Expression Regulation, Developmental
- RNA-Binding Proteins
- Life Sciences
- Oligonucleotides, Antisense
- Zebrafish Proteins
- Ribosome
- Germ Cells
- RNA Methylation and Modification in Gene Expression
- Embryo
- Gene Knockdown Techniques
- FOS: Biological sciences
- RNA
- Gene expression
- CRISPR-Cas Systems
- Tumor Suppressor Protein p53
- Yolk sac
詳細情報 詳細情報について
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- CRID
- 1361412893260683136
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- ISSN
- 20452322
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- PubMed
- 31792295
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE