Tadalafil, a phosphodiesterase type 5 inhibitor, restores urethra and detrusor function in the initial phase of diabetes in rats
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- Daisuke Gotoh
- Department of Urology Nara Medical University Kashihara Japan
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- Kazumasa Torimoto
- Department of Urology Nara Medical University Kashihara Japan
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- Hirotaka Iwasaki
- Department of Pharmacology Shiga University of Medical Science Otsu Japan
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- Takashi Iwamoto
- Department of Urology Nara Medical University Kashihara Japan
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- Yosuke Morizawa
- Department of Urology Nara Medical University Kashihara Japan
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- Shunta Hori
- Department of Urology Nara Medical University Kashihara Japan
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- Makito Miyake
- Department of Urology Nara Medical University Kashihara Japan
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- Nobumichi Tanaka
- Department of Urology Nara Medical University Kashihara Japan
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- Akihide Hirayama
- Department of Urology Kindai Nara Hospital Ikoma Japan
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- Eiichiro Nishi
- Department of Pharmacology Shiga University of Medical Science Otsu Japan
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- Kiyohide Fujimoto
- Department of Urology Nara Medical University Kashihara Japan
書誌事項
- 公開日
- 2019-06-17
- 資源種別
- journal article
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1111/luts.12272
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:sec><jats:title>Objective</jats:title><jats:p>This study investigated the effects of tadalafil on the urethra and detrusor in the initial phase of diabetes in rats.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Thirty‐six female Sprague‐Dawley rats were assigned to a non‐diabetes (ND), diabetes (D), or tadalafil‐treated diabetes (DT) group (n = 12 per group), with the DT group receiving oral tadalafil (2 mg/kg/d) for 7 days before the experiments. Seven weeks after diabetes induction (by a single intraperitoneal injection of streptozotocin), urethral and intravesical pressure were simultaneously recorded in vivo, whereas responses of detrusor strips to potassium chloride (30 mM), electrical field stimulation (EFS) and carbachol were measured in vitro.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>The intravesical pressure at which the urethra started to relax was significantly lower in the DT than D group (mean [± s.d.] 18.9 ± 2.9 vs 29.1 ± 6.6 cm H<jats:sub>2</jats:sub>O; <jats:italic>P</jats:italic> < .05). In addition, the reduction in urethral pressure was significantly larger in the DT than D group (−10.9 ± 4.0 vs −4.0 ± 2.9 cm H<jats:sub>2</jats:sub>O; <jats:italic>P</jats:italic> < .05). Detrusor stimulation revealed that the mean contractile responses to EFS and carbachol were significantly lower in the ND and DT groups than in the D group (120.7 ± 26.5% and 130.8 ± 15.8% vs 200.1 ± 47.9% of the 30 mM KCl‐induced contraction, respectively, in response to 50 Hz EFS [<jats:italic>P</jats:italic> < .05]; 211.1 ± 35.4% and 208.4 ± 25.3% vs 425.7 ± 125.0% of the 30 mM KCl‐induced contraction, respectively, in response to 10<jats:sup>−3</jats:sup> M carbachol [<jats:italic>P</jats:italic> < .05]).</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>Tadalafil restored urethral relaxation function and detrusor responses to EFS and carbachol during the initial phase of diabetes.</jats:p></jats:sec>
収録刊行物
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- LUTS: Lower Urinary Tract Symptoms
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LUTS: Lower Urinary Tract Symptoms 11 (4), 241-247, 2019-06-17
Wiley
