Xyloglucan Fucosyltransferase, an Enzyme Involved in Plant Cell Wall Biosynthesis

DOI Web Site 被引用文献6件
  • Robyn M. Perrin
    Michigan State University–Department of Energy (MSU-DOE) Plant Research Laboratory,
  • Amy E. DeRocher
    Michigan State University–Department of Energy (MSU-DOE) Plant Research Laboratory,
  • Maor Bar-Peled
    Michigan State University–Department of Energy (MSU-DOE) Plant Research Laboratory,
  • Weiqing Zeng
    Michigan State University–Department of Energy (MSU-DOE) Plant Research Laboratory,
  • Lorena Norambuena
    Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile.
  • Ariel Orellana
    Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile.
  • Natasha V. Raikhel
    Michigan State University–Department of Energy (MSU-DOE) Plant Research Laboratory,
  • Kenneth Keegstra
    Michigan State University–Department of Energy (MSU-DOE) Plant Research Laboratory,

書誌事項

公開日
1999-06-18
DOI
  • 10.1126/science.284.5422.1976
公開者
American Association for the Advancement of Science (AAAS)

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説明

<jats:p> Cell walls are crucial for development, signal transduction, and disease resistance in plants. Cell walls are made of cellulose, hemicelluloses, and pectins. Xyloglucan (XG), the principal load-bearing hemicellulose of dicotyledonous plants, has a terminal fucosyl residue. A 60-kilodalton fucosyltransferase (FTase) that adds this residue was purified from pea epicotyls. Peptide sequence information from the pea FTase allowed the cloning of a homologous gene, <jats:italic>AtFT1</jats:italic> , from <jats:italic>Arabidopsis</jats:italic> . Antibodies raised against recombinant AtFTase immunoprecipitate FTase enzyme activity from solubilized <jats:italic>Arabidopsis</jats:italic> membrane proteins, and <jats:italic>AtFT1</jats:italic> expressed in mammalian COS cells results in the presence of XG FTase activity in these cells. </jats:p>

収録刊行物

  • Science

    Science 284 (5422), 1976-1979, 1999-06-18

    American Association for the Advancement of Science (AAAS)

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