Sequence Variations Among 17 New Radish Isolates of <i>Turnip mosaic virus</i> Showing Differential Pathogenicity and Infectivity in <i>Nicotiana benthamiana</i>, <i>Brassica rapa</i>, and <i>Raphanus sativus</i>
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- Junsu Gong
- Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea;
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- Hye-Kyoung Ju
- Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea;
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- Ik-Hyun Kim
- Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea;
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- Eun-Young Seo
- Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea;
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- In-Sook Cho
- Rural Development Administration, National Institute of Horticultural and Herbal Science, 100 Jeollabuk-do, Korea;
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- Wen-Xing Hu
- Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea;
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- Jae-Yeong Han
- Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea;
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- Jung-Kyu Kim
- Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea;
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- Su Ryun Choi
- Department of Horticulture, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea; and
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- Young Pyo Lim
- Department of Horticulture, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea; and
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- John Hammond
- Floral and Nursery Plants Research Unit, U.S. National Arboretum, U.S. Department of Agriculture Agricultural Research Service, Beltsville, MD 20705, U.S.A.
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- Hyoun-Sub Lim
- Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea;
抄録
<jats:p> Infectious clones were generated from 17 new Korean radish isolates of Turnip mosaic virus (TuMV). Phylogenetic analysis indicated that all new isolates, and three previously characterized Korean radish isolates, belong to the basal-BR group (indicating that the pathotype can infect both Brassica and Raphanus spp.). Pairwise analysis revealed genomic nucleotide and polyprotein amino acid identities of >87.9 and >95.7%, respectively. Five clones (HJY1, HJY2, KIH2, BE, and prior isolate R007) had lower sequence identities than other isolates and produced mild symptoms in Nicotiana benthamiana. These isolates formed three distinct sequence classes (HJY1/HJY2/R007, KIH2, and BE), and several differential amino acid residues (in P1, P3, 6K2, and VPg) were present only in mild isolates HJY1, HJY2, and R007. The remaining isolates all induced systemic necrosis in N. benthamiana. Four mild isolates formed a phylogenetic subclade separate from another subclade including all of the necrosis-inducing isolates plus mild isolate KIH2. Symptom severity in radish and Chinese cabbage genotypes was not correlated with pathogenicity in N. benthamiana; indeed, Chinese cabbage cultivar Norang was not infected by any isolate, whereas Chinese cabbage cultivar Chusarang was uniformly susceptible. Four isolates were unable to infect radish cultivar Iljin, but no specific amino acid residues were correlated with avirulence. These results may lead to the identification of new resistance genes against TuMV. </jats:p>
収録刊行物
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- Phytopathology®
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Phytopathology® 109 (5), 904-912, 2019-05
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